Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (C-Term), (AA 486-499) antibody

Details for Product No. ABIN117946
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Synonyms X-FZY, ba276h19.3, cdc20a, fizzy1, p55cdc, CDC20A, bA276H19.3, p55CDC, 2310042N09Rik, C87100
C-Term, AA 486-499
(20), (7), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
(67), (20), (19), (4), (2), (2), (2), (2)
(62), (9)
(2), (2), (2), (2), (2), (2)
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(65), (34), (20), (16), (11), (9), (5), (3), (2), (1), (1), (1)
Pubmed 8 references available
Quantity 0.1 mL
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Catalog No. ABIN117946
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Immunogen Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.
Sequence Human CDC20 (fizzy) (499 aa 54.7 kDa): 1 maqfafesdl hsllqldapi pnapparwqr kakeaagpap spmraanrsh sagrtpgrtp 61 gkssskvqtt pskpggdryi phrsaaqmev asfllskenq sensqtptkk ehqkawalnl 21 ngfdveeaki lrlsgkpqna pegyqnrlkv lysqkatpgs srktcryips lpdrildape 181 irndyylnlv dwssgnvlav aldnsvylws assgdilqll qmeqpgeyis svawikegny 241 lavgtssaev qlwdvqqqkr lrnmtshsar vgslswnsyi lssgsrsghi hhhdvrvaeh 301 hvatlsghsq evcglrwapd grhlasggnd nlvnvwpsap geggwvplqt ftqhqgavka 361 vawcpwqsnv latgggtsdr hiriwnvcsg aclsavdahs qvcsilwsph ykelisghgf 421 aqnqlviwky ptmakvaelk ghtsrvlslt mspdgatvas aaadetlrlw rcfeldparr 481 rerekasaak sslihqgir
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.
Purification Delipidation and defibrination.
Alternative Name CDC20
Background CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.Synonyms: Cell division cycle protein 20 homolog, p55CDC
Gene ID 991
NCBI Accession NP_001246
UniProt Q12834
Research Area Cell Cycle
Application Notes Western blot: This antibody reacts with human CDC20 (fizzy) (1: 500 - 1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa bandcorresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressingCDC20 can be used as a positive control.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
Supplier Images
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (C-Term), (AA 486-499) antibody Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto- tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
Background publications Song, Song, Ayad et al.: "The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex." in: Nature cell biology, Vol. 6, Issue 2, pp. 129-37, 2004 (PubMed).

Zur, Brandeis: "Timing of APC/C substrate degradation is determined by fzy/fzr specificity of destruction boxes." in: The EMBO journal, Vol. 21, Issue 17, pp. 4500-10, 2002 (PubMed).

Asadi, Jörgensen, Jacobsson: "Elovl1 and p55Cdc genes are localized in a tail-to-tail array and are co-expressed in proliferating cells." in: The Journal of biological chemistry, Vol. 277, Issue 21, pp. 18494-500, 2002 (PubMed).

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).

Weinstein, Karim, Geschwind et al.: "Genomic organization, 5' flanking enhancer region, and chromosomal assignment of the cell cycle gene, p55Cdc." in: Molecular genetics and metabolism, Vol. 64, Issue 1, pp. 52-7, 1998 (PubMed).

Weinstein: "Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28501-11, 1997 (PubMed).

Weinstein, Jacobsen, Hsu-Chen et al.: "A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3350-63, 1994 (PubMed).

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