Ras-Like Without CAAX 2 (RIT2) (C-Term), (AA 102-113) antibody

Details for Product No. ABIN117958
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Antigen
Synonyms RIT2, RIBA, Rin, Roc2, RIN, ROC2, Rit1, CKBBP1, SAG
Epitope
C-Term, AA 102-113
(9), (8), (5), (2), (2), (1), (1)
Reactivity
Caenorhabditis elegans (C. elegans), Human, Mouse (Murine), Zebrafish (Danio rerio)
(36), (4), (1), (1), (1)
Host
Rabbit
(19), (17)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(36), (11), (8), (5), (4), (3), (2), (2), (2)
Pubmed 14 references available
Quantity 0.1 mL
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Catalog No. ABIN117958
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Immunogen Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.
Sequence Human ROC2 (113 aa 12.6 kDa) 1 madvedgeet calashsgss gsksggdkmf slkkwnavam wswdvecdtc aicrvqvmda 61 clrcqaenkq edcvvvwgec nhsfhnccms lwvkqnnrcp lcqqdwvvqr igk
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC2. Cross reactivity may also occur with ROC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and APC11.
Purification Delipidation and defibrination.
Alternative Name ROC2
Background ROC1 also known as RING-box protein 2, Rbx2, RING finger protein 7, Regulator of cullins 2, CKII betabinding protein 1, and CKBBP1, is a probable component of the SCF (SKP1-CUL1-F- box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC2 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. ROC2 may play a role in protecting cells from apoptosis induced by redox agents. ROC2 has a cytoplasmic and nuclear localization and is expressed in heart, liver, skeletal muscle and pancreas tissues, and at very low levels in brain, placenta and lung. 1,10-phenanthroline induces ROC2 expression. The RING-type zinc finger domain is essential for ubiquitin ligase activity. Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.
Alternate names: GL2-2, GLABRA 2-like homeobox protein 2, HD-ZIP protein ROC2, Homeobox-leucine zipper protein ROC2, Homeodomain transcription factor ROC2, Protein RICE OUTERMOST CELL- SPECIFIC 2
Gene ID 4337074
NCBI Accession NP_001053951
UniProt Q0J9X2
Application Notes This antibody reacts with human ROC2 by Western blot (1: 500-1: 1,000) andImmunoprecipitation. The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins does occur. A 12.6 kDa band corresponding tohuman ROC2 is detected. Most cell lines expressing ROC2 can be used as a positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
Expiry Date 12 months
Supplier Images
anti-Ras-Like Without CAAX 2 (RIT2) (C-Term), (AA 102-113) antibody Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto- tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
Background publications Duan, Wang, Aviram et al.: "SAG, a novel zinc RING finger protein that protects cells from apoptosis induced by redox agents." in: Molecular and cellular biology, Vol. 19, Issue 4, pp. 3145-55, 1999 (PubMed).

Kamura, Koepp, Conrad et al.: "Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase." in: Science (New York, N.Y.), Vol. 284, Issue 5414, pp. 657-61, 1999 (PubMed).

Skowyra, Koepp, Kamura et al.: "Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1." in: Science (New York, N.Y.), Vol. 284, Issue 5414, pp. 662-5, 1999 (PubMed).

Tan, Fuchs, Chen et al.: "Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha." in: Molecular cell, Vol. 3, Issue 4, pp. 527-33, 1999 (PubMed).

Ohta, Michel, Schottelius et al.: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, 1999 (PubMed).

Seol, Feldman, Zachariae et al.: "Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34." in: Genes & development, Vol. 13, Issue 12, pp. 1614-26, 1999 (PubMed).

Son, Park, Kim et al.: "Protein kinase CKII interacts with and phosphorylates the SAG protein containing ring-H2 finger motif." in: Biochemical and biophysical research communications, Vol. 263, Issue 3, pp. 743-8, 1999 (PubMed).

Swaroop, Wang, Miller et al.: "Yeast homolog of human SAG/ROC2/Rbx2/Hrt2 is essential for cell growth, but not for germination: chip profiling implicates its role in cell cycle regulation." in: Oncogene, Vol. 19, Issue 24, pp. 2855-66, 2000 (PubMed).

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Furukawa, Zhang, McCarville et al.: "The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1." in: Molecular and cellular biology, Vol. 20, Issue 21, pp. 8185-97, 2000 (PubMed).

Swaroop, Gosink, Sun: "SAG/ROC2/Rbx2/Hrt2, a component of SCF E3 ubiquitin ligase: genomic structure, a splicing variant, and two family pseudogenes." in: DNA and cell biology, Vol. 20, Issue 7, pp. 425-34, 2001 (PubMed).

Kim, Lee, Son et al.: "Phosphorylation of threonine 10 on CKBBP1/SAG/ROC2/Rbx2 by protein kinase CKII promotes the degradation of IkappaBalpha and p27Kip1." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28462-9, 2003 (PubMed).

General Jung, Park, Pae et al.: "[Short-term therapy with pegylated interferon plus ribavirin for the chronic hepatitis C genotype 2 patients]" in: The Korean journal of hepatology, Vol. 13, Issue 3, pp. 341-8, 2007 (PubMed).

Kim, Choi, Park et al.: "Endometrial stromal sarcoma of the small bowel: a case report and review of literature." in: Annals of diagnostic pathology, Vol. 12, Issue 2, pp. 128-33, 2008 (PubMed).

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