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MAD2 Mitotic Arrest Deficient-Like 2 (Yeast) (MAD2L2) (AA 3-14) antibody

Details for Product No. ABIN117980
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Antigen
Synonyms mad2b, 2310033C13Rik, MAD2B, REV7, mad2B, mad2l2-A, xMAD2L2, POLZ2, mad2l2, zgc:110299
Epitope
AA 3-14
(5), (5), (1)
Reactivity
Human
(25), (14), (14), (11), (11), (11), (11)
Host
Rabbit
(23), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(16), (9), (6), (5), (4), (3), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN117980
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2.
Specificity MAD2L2 Species Reactivity: Tested: Human.
Purification Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Alternative Name MAD2L2
Background MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.
Alternate names: MAD2B, Mitotic spindle assembly checkpoint protein MAD2B, REV7
Gene ID 10459
NCBI Accession NP_001120797.1
UniProt Q9UI95
Application Notes This affinity purified antibody has been tested for use in ELISA (1: 7,500-1: 30,000) againstthe immunizing peptide and by Western blotting (1: 500-1: 2,000) against HeLa whole celllysates and nuclear extracts. Reactivity in other immunoassays is unknown. Antibodyactivity can be blocked using a control peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.3 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Supplier Images
anti-MAD2 Mitotic Arrest Deficient-Like 2 (Yeast) (MAD2L2) (AA 3-14) antibody Immunoblotting. Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
Background publications Chen, Fang: "MAD2B is an inhibitor of the anaphase-promoting complex." in: Genes & development, Vol. 15, Issue 14, pp. 1765-70, 2001 (PubMed).

General Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).

Nelson, Schloendorff, Blobel: "Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta." in: The Biochemical journal, Vol. 343 Pt 3, pp. 673-80, 2000 (PubMed).

Murakumo, Roth, Ishii et al.: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, 2000 (PubMed).

Validation Images
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