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Details for Product No. ABIN117980

MAD2 Mitotic Arrest Deficient-Like 2 (Yeast) (MAD2L2) (AA 3-14) antibody

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Antigen
Synonyms MAD2B, POLZ2, REV7, 2310033C13Rik, mad2B, mad2l2-A, xMAD2L2, mad2b
Epitope
»Alternatives AA 3-14
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 4 references available
Catalog no. ABIN117980
Quantity 0.1 mg
Price
379.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2.
Specificity MAD2L2 Species Reactivity: Tested: Human.
Purification Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Alternative Name MAD2L2
Background MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.
Alternate names: MAD2B, Mitotic spindle assembly checkpoint protein MAD2B, REV7
Gene ID 10459
NCBI Accession NP_001120797.1
UniProt Q9UI95
Application Notes This affinity purified antibody has been tested for use in ELISA (1: 7,500-1: 30,000) againstthe immunizing peptide and by Western blotting (1: 500-1: 2,000) against HeLa whole celllysates and nuclear extracts. Reactivity in other immunoassays is unknown. Antibodyactivity can be blocked using a control peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.3 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C
Storage Comment Store undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Background publications Chen, Fang: "MAD2B is an inhibitor of the anaphase-promoting complex." in: Genes & development, Vol. 15, Issue 14, pp. 1765-70, 2001 (PubMed).

Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).

Nelson, Schloendorff, Blobel: "Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta." in: The Biochemical journal, Vol. 343 Pt 3, pp. 673-80, 2000 (PubMed).

Murakumo, Roth, Ishii et al.: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, 2000 (PubMed).

Alternatives for antigen "MAD2 Mitotic Arrest Deficient-Like 2 (Yeast) (MAD2L2)", type "Antibodies"
Hosts (21), (2)
Reactivities (23), (13), (13), (11), (11), (11), (11)
Applications (13), (9), (8), (5), (4), (3), (1), (1), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (4)
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