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MAD2 Mitotic Arrest Deficient-Like 2 (Yeast) (MAD2L2) (AA 3-14) antibody

Details for Product No. ABIN117980, Supplier: Log in to see
Antigen
  • mad2b
  • 2310033C13Rik
  • MAD2B
  • REV7
  • mad2B
  • mad2l2-A
  • xMAD2L2
  • POLZ2
  • mad2l2
  • zgc:110299
Epitope
AA 3-14
9
7
4
1
1
1
Reactivity
Human
39
17
17
1
Host
Rabbit
34
5
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
28
11
11
7
7
7
3
1
1
Supplier
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2.
Specificity MAD2L2
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Alternative Name MAD2L2 (MAD2L2 Antibody Abstract)
Background MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.Synonyms: MAD2B, Mitotic spindle assembly checkpoint protein MAD2B, REV7
Gene ID 10459
NCBI Accession NP_001120797
UniProt Q9UI95
Pathways
Application Notes This affinity purified antibody has been tested for use in ELISA (1: 7,500-1: 30,000) againstthe immunizing peptide and by Western blotting (1: 500-1: 2,000) against HeLa whole celllysates and nuclear extracts. Reactivity in other immunoassays is unknown. Antibodyactivity can be blocked using a control peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.3 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C. DO NOT FREEZE!
Supplier Images
Western Blotting (WB) image for anti-MAD2 Mitotic Arrest Deficient-Like 2 (Yeast) (MAD2L2) (AA 3-14) antibody (ABIN117980) Immunoblotting. Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to ...
Background publications Chen, Fang: "MAD2B is an inhibitor of the anaphase-promoting complex." in: Genes & development, Vol. 15, Issue 14, pp. 1765-70, 2001 (PubMed).

Murakumo, Roth, Ishii et al.: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, 2000 (PubMed).

Nelson, Schloendorff, Blobel: "Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta." in: The Biochemical journal, Vol. 343 Pt 3, pp. 673-80, 2000 (PubMed).

Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).