LIM Domain Binding 2 (LDB2) (AA 107-120) antibody

Details for Product No. ABIN118016
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Synonyms LDB2, CLIM-1, CLIM1, LDB1, AI035351, AW146358, CLP-36, Ldb3, clim1, clim1b, ldb2
AA 107-120
(17), (14), (10), (7), (4), (4), (2), (1), (1), (1), (1)
Chicken, Human, Mouse (Murine)
(42), (14), (3), (3), (3), (2), (2), (1), (1)
(36), (14)
(3), (3), (3), (3), (3), (3)
Enzyme Immunoassay (EIA)
(46), (30), (16), (12), (6), (6), (3), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN118016
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 107-120 of Mouse LDB2.
Sequence S-K-E-S-Y-H-N-S-S-I-T-V-D
Specificity This affinity purified antibody is directed against mouse LDB2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100 % homology for the immunogen sequence. Cross reactivity with LDB2 homologues from other sources has not been determined.
Purification Immunoaffinity chromatography.
Alternative Name LDB2
Background LDB2 (LIM homeobox protein cofactor CLIM-1a). It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1. LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95 % , 73 % and 62 % amino acid identity with mouse Ldb1, respectively.Synonyms: CLIM-1, CLIM1, Carboxyl-terminal LIM domain-binding protein 1, LDB2, LIM domain-binding factor CLIM1, LIM domain-binding protein 2
Gene ID 16826
NCBI Accession NP_001070866
UniProt O55203
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes This affinity purified antibody has been tested for use in ELISA (1: 5,000-1: 20,000)andWestern Blot (1: 500-1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 43 kDa in size corresponding to LDB2 by westernblotting in the appropriate cell lysate or extract. Immunofluorescence microscopy.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.65 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-LIM Domain Binding 2 (LDB2) (AA 107-120) antibody Figure 1. Western blot using Affinity Purified anti-LDB2 antibody shows detection of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Background publications Ostendorff, Peirano, Peters et al.: "Ubiquitination-dependent cofactor exchange on LIM homeodomain transcription factors." in: Nature, Vol. 416, Issue 6876, pp. 99-103, 2002 (PubMed).

Semina, Altherr, Murray: "Cloning and chromosomal localization of two novel human genes encoding LIM-domain binding factors CLIM1 and CLIM2/LDB1/NLI." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 9, Issue 11, pp. 921-4, 1998 (PubMed).

Toyama, Kobayashi, Tomita et al.: "Expression of LIM-domain binding protein (ldb) genes during zebrafish embryogenesis." in: Mechanisms of development, Vol. 71, Issue 1-2, pp. 197-200, 1998 (PubMed).

Bach, Carrière, Ostendorff et al.: "A family of LIM domain-associated cofactors confer transcriptional synergism between LIM and Otx homeodomain proteins." in: Genes & development, Vol. 11, Issue 11, pp. 1370-80, 1997 (PubMed).

General Shimizu, Shimizu, Kinebuchi et al.: "[A case of acute eosinophilic pneumonia probably induced by minocycline]" in: Nihon Koky?ki Gakkai zasshi = the journal of the Japanese Respiratory Society, Vol. 46, Issue 2, pp. 136-40, 2008 (PubMed).

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