CD6 (CD6) antibody

Details for Product No. ABIN119554
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Antigen
Synonyms TP120, OX52
Reactivity
Human
(106), (31), (19), (8), (5)
Host
Mouse
(101), (32), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(16), (11), (9), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(106), (53), (34), (23), (17), (16), (14), (12), (9), (5), (2), (1)
Pubmed 7 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN119554
308.00 $
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Immunogen Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone).
Clone MEM-98
Isotype IgG1
Specificity The antibody reacts with CD6, a 100-130 kD single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.
Purification Protein-A affinity chromatography
Purity > 95 % pure (by SDS-PAGE)
Alternative Name CD6
Background CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.
Alternate names: T-cell differentiation antigen CD6, T12, TP120
Gene ID 923
NCBI Accession NP_006716
UniProt P30203
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Immunoprecipitation. Flow Cytometry. Western Blotting under non reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 m g/ml, Positive tissue: spleen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Bazil, Stefanová, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).

Osorio, De Santiago, Aguilar-Santelises et al.: "CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM." in: Blood, Vol. 89, Issue 8, pp. 2833-41, 1997 (PubMed).

Zimmerman, Joosten, Torensma et al.: "Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells." in: Blood, Vol. 107, Issue 8, pp. 3212-20, 2006 (PubMed).

Hassan, Simmonds, Clarkson et al.: "CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6727-38, 2006 (PubMed).

Castro, Oliveira, Nunes et al.: "Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4351-61, 2007 (PubMed).

May, Law, Fitzgibbon et al.: "Software platform for rapidly creating computational tools for mass spectrometry-based proteomics." in: Journal of proteome research, Vol. 8, Issue 6, pp. 3212-7, 2009 (PubMed).

Xie, Xu, Leal-Ortiz et al.: "Nanostraw-electroporation system for highly efficient intracellular delivery and transfection." in: ACS nano, Vol. 7, Issue 5, pp. 4351-8, 2013 (PubMed).

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