Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody

Details for Product No. ABIN120000
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Synonyms ITGA5, Cd49e, Fnra, VLA5, CD49e, FNRA, VLA5A, Itga5_retired
Mouse (Murine)
(111), (107), (35), (13), (12), (3), (1)
(62), (52), (45), (37), (1)
Clonality (Clone)
Monoclonal ()
(32), (19), (13), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(130), (71), (47), (40), (35), (27), (15), (13), (10), (10), (6), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN120000
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Immunogen Mouse MC/9 cell line. Spleen cells from immunised Lewis rats were fused with cells of the Murine P3X63Ag8.653
Clone MFR5 (5H10)
Isotype IgG2a
Specificity This antibody recognises murine integrin alpha 5 (CD49e), which associates with the beta 1 integrin (CD29) to form the fibronectin receptor CD49e/CD29 (VLA-5). Clone MFR5(5H10) has been reported to block CD49e mediated function. We recommend the use of SM2100LE for this purpose.
Purification Affinity Chromatography on Protein G.
Alternative Name CD49e / ITGA5
Background VLA-5 plays an important role in cell adhesion and migration and is expressed by mast cells, thymocytes, Tcells and monocytes.
Alternate names: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5
Gene ID 10090
UniProt P11688
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry (1/10-1/25): Use 10 ul of the suggested working dilution to label 10e6 cells in 100 ul. Immunohistochemitry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD49e (ABIN120000).
Background publications Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995 (PubMed).

General Cukierman, Pankov, Stevens et al.: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, 2001 (PubMed).

Validation Images
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