CD163 Molecule (CD163) antibody

Details for Product No. ABIN120083
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Antigen
Synonyms DMBT1, CD163, CD163v2, CD163v3, M130, MM130
Reactivity
Human, Monkey, Sheep (Ovine)
(140), (36), (24), (22), (17), (17), (4)
Host
Mouse
(112), (57), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(23), (16), (14), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(85), (65), (63), (46), (28), (20), (19), (14), (13), (10), (8), (6), (2)
Pubmed 11 references available
Quantity 0.2 mg
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Catalog No. ABIN120083
720.50 $
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Immunogen Leucocytes harvested from pleural cavity of patients with idiopathic spontaneous pneumothorax.
Clone EDHu-1
Isotype IgG1
Specificity This antibody recognises the CD163 cell surface antigen. Clone EDHu-1 is reported to inhibit the binding of haptoglobin:haemoglobin to CD163. Species Reactivity: Tested: Human, Rhesus Monkey, Sheep.
Purification Affinity Chromatography on Protein A
Alternative Name CD163
Background CD163 is a 130-140kD glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone. It is a member of the scavenger receptor cysteine- rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant.
Alternate names: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine- rich type 1 protein M130
Gene ID 9332
NCBI Accession NP_004235
UniProt Q86VB7
Research Area Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells
Application Notes Flow Cytometry. Immunohistochemistry on frozen sections. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 0.09% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Kristiansen, Graversen, Jacobsen et al.: "Identification of the haemoglobin scavenger receptor." in: Nature, Vol. 409, Issue 6817, pp. 198-201, 2001 (PubMed).

Madsen, Møller, Nielsen et al.: "Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 51561-7, 2004 (PubMed).

Montes de Oca, Torres, De Sanctis et al.: "Skeletal muscle inflammation and nitric oxide in patients with COPD." in: The European respiratory journal, Vol. 26, Issue 3, pp. 390-7, 2005 (PubMed).

Kim, Alvarez, Fisher et al.: "CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood." in: The American journal of pathology, Vol. 168, Issue 3, pp. 822-34, 2006 (PubMed).

Keh, Onyekwelu, McManus et al.: "Corrosive injury to upper gastrointestinal tract: Still a major surgical dilemma." in: World journal of gastroenterology : WJG, Vol. 12, Issue 32, pp. 5223-8, 2006 (PubMed).

Fabriek, Polfliet, Vloet et al.: "The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor." in: Blood, Vol. 109, Issue 12, pp. 5223-9, 2007 (PubMed).

Grund, Schittenhelm, Roser et al.: "The microglial/macrophagic response at the tumour-brain border of invasive meningiomas." in: Neuropathology and applied neurobiology, Vol. 35, Issue 1, pp. 82-8, 2009 (PubMed).

Moreno, Muñoz-García, Martín-Ventura et al.: "The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis." in: Atherosclerosis, Vol. 207, Issue 1, pp. 103-10, 2009 (PubMed).

Jensen, Schmidt, Møller et al.: "Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 27, Issue 20, pp. 3330-7, 2009 (PubMed).

Taus, Schneider, Oaks et al.: "Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo." in: Veterinary microbiology, Vol. 145, Issue 1-2, pp. 47-53, 2010 (PubMed).

Vinet-Oliphant, Alvarez, Buza et al.: "Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS." in: The American journal of pathology, Vol. 177, Issue 3, pp. 1286-97, 2010 (PubMed).

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