Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody

Antigen: Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1)

Synonyms: KLR, CD314, NKG2D, NKG2-D, FLJ17759, FLJ75772, D12S2489E, KLRK1, Nkrp2, Nkg2d, D6H12S2489E

Clonality: Monoclonal (1D11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN120294

Additional Information

Alternative name: CD314 / KLRK1

UniProt: P26718

Immunogen: NKL cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line

Cross-Reactivity: Human

Isotype: IgG1

Clone: 1D11

Description: CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding toCD314 results in NK cell activation and potent costimulation of effector T cells. Alternate Names: D12S2489E, NKG2D, KLRK1, NKG2-D-activating NK Receptor, NK Cell Receptor D, Killer cell lectin- like receptor subfamily K member 1, NKG2-D type II integral membrane protein.

Specificity: This antibody is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). Clone 1D11 is reported to inhibit T cell recognition of MICA (1,6). We recommend the use of ABIN120293 for such studies.

Application Details

Application Notes: Flow Cytometry: Use 10 ul of 1/100 diluted antibody to label 106 cells in 100 ul. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD314 (ABIN120294)

Publications

Bauer, Groh, Wu et al.: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Wu, Song, Bakker et al.: "An activating immunoreceptor complex formed by NKG2D and DAP10." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 730-2, 1999 (PubMed).

Groh, Rhinehart, Randolph-Habecker et al.: "Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells." in: Nature immunology, Vol. 2, Issue 3, pp. 255-60, 2001 (PubMed).

Groh, Bruhl, El-Gabalawy et al.: "Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 16, pp. 9452-7, 2003 (PubMed).

Jinushi, Takehara, Tatsumi et al.: "Autocrine/paracrine IL-15 that is required for type I IFN-mediated dendritic cell expression of MHC class I-related chain A and B is impaired in hepatitis C virus infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 10, pp. 5423-9, 2003 (PubMed).

Das, Sugita, Brenner: "Mechanisms of Vdelta1 gammadelta T cell activation by microbial components." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6578-86, 2004 (PubMed).