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Details for Product No. ABIN120294

Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody

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Antigen
Synonyms CD314, D12S2489E, KLR, NKG2-D, NKG2D, Nkrp2, KLRK1, Nkg2d, D6H12S2489E
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Flow Cytometry (FACS)
Pubmed 6 references available
Catalog no. ABIN120294
Quantity 0.2 mg
Price
489.50 $   Plus shipping costs $45.00
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Immunogen NKL cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line.
Clone 1D11
Isotype IgG1
Specificity This antibody is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). Clone 1D11 is reported to inhibit T cell recognition of MICA (1,6). We recommend the use of SM2260LE for such studies.
Purification Affinity Chromatography on Protein G.
Alternative Name CD314 / KLRK1
Background CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding toCD314 results in NK cell activation and potent costimulation of effector T cells.
Alternate names: D12S2489E, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2D
Gene ID 100528032
NCBI Accession NP_001186734.1
UniProt P26718
Application Notes Flow Cytometry: Use 10 ul of 1/100 diluted antibody to label 106 cells in 100 ul. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Bauer, Groh, Wu et al.: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Wu, Song, Bakker et al.: "An activating immunoreceptor complex formed by NKG2D and DAP10." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 730-2, 1999 (PubMed).

Groh, Rhinehart, Randolph-Habecker et al.: "Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells." in: Nature immunology, Vol. 2, Issue 3, pp. 255-60, 2001 (PubMed).

Groh, Bruhl, El-Gabalawy et al.: "Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 16, pp. 9452-7, 2003 (PubMed).

Jinushi, Takehara, Tatsumi et al.: "Autocrine/paracrine IL-15 that is required for type I IFN-mediated dendritic cell expression of MHC class I-related chain A and B is impaired in hepatitis C virus infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 10, pp. 5423-9, 2003 (PubMed).

Das, Sugita, Brenner: "Mechanisms of Vdelta1 gammadelta T cell activation by microbial components." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6578-86, 2004 (PubMed).

Alternatives for antigen "Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1)", type "Antibodies"
Hosts (46), (45), (32), (27), (9), (1)
Reactivities (94), (81), (20)
Applications (134), (29), (27), (21), (13), (10), (8), (4), (4), (4), (3), (1)
Conjugates (20), (17), (13), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (4)
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