CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB) (AA 1-271), (N-Term) antibody

Details for Product No. ABIN121153
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Antigen
Synonyms
C/EBPbeta, c/ebp-beta, crp2, il6dbp, lap, nf-il6, tcf5, xC/EBPbeta, cebpbb, cb380, c/ebpb, fe11e05, wu:fe11e05, CEBPB, LOC100136165, C/EBP-beta, CRP2, IL6DBP, LAP, NF-IL6, TCF5, IL-6DBP, LIP, NF-M, Nf ... show more
C/EBPbeta, c/ebp-beta, crp2, il6dbp, lap, nf-il6, tcf5, xC/EBPbeta, cebpbb, cb380, c/ebpb, fe11e05, wu:fe11e05, CEBPB, LOC100136165, C/EBP-beta, CRP2, IL6DBP, LAP, NF-IL6, TCF5, IL-6DBP, LIP, NF-M, Nfil6, Il6dbp show less
Epitope
AA 1-271, N-Term
(20), (13), (11), (10), (8), (6), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(105), (79), (59), (26), (26), (12), (12), (1)
Host
Mouse
(93), (34), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(94), (41), (30), (21), (19), (11), (9), (7), (5), (4), (3), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mL
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Catalog No. ABIN121153
412.50 $
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Immunogen Recombinant human C/EBP-beta (1-271 aa) purified from E. coli
Clone 47A1
Isotype IgG1
Purification Protein-G affinity chromatography
Alternative Name CEBPB
Background CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPα, C/EBPβ, C/EBPδ, C/EBPε, and C/EBPγ. Among these proteins, C/EBPβ is usually highly expressed in liver, intestine, lung and adipose. C/EBPβ has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.Synonyms: C/EBP beta, CCAAT/enhancer-binding protein beta, LAP, Liver activator protein, NF-IL6, Nuclear factor NF-IL6, TCF5, Transcription factor 5
UniProt P17676
Application Notes ELISA. Western blot (1/500 - 1/2,000), recommended starting dilution is 1:1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB) (AA 1-271), (N-Term) antibody Western blot analysis.,.NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-beta antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
General Lekstrom-Himes, Xanthopoulos: "Biological role of the CCAAT/enhancer-binding protein family of transcription factors." in: The Journal of biological chemistry, Vol. 273, Issue 44, pp. 28545-8, 1998 (PubMed).

Parkin, Baer, Copeland et al.: "Regulation of CCAAT/enhancer-binding protein (C/EBP) activator proteins by heterodimerization with C/EBPgamma (Ig/EBP)." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23563-72, 2002 (PubMed).

Su, Chou, Chang et al.: "Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51150-8, 2003 (PubMed).

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