p28 ING5 (AA 127-140) antibody

Antigen: p28 ING5

Binding Site: AA 127-140

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN129552

Additional Information

Gene ID: 84289, AAH05370 (isoform 2)

UniProt: Q8WYH8

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence shows homology to and 2 for ING5.

Format: Liquid

Isotype: IgG

Description: P28 ING5 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.  Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) encodes the longest isoform.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kDa or 26 kDa in size corresponding to variants of ING5 by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:4,000 - 1:16,000
Western Blot Dilution: 1:500 - 1:1,000

Concentration: 1.52 mg/mL

Purification: Sterile filtered

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Restrictions: For Research Use only

Images

Western blot analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time. Other detection systems will yield similar results.

Western blot analysis is shown using antibodies-online's Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4

Publications

Iizuka, Sarmento, Sekiya et al.: "Hbo1 Links p53-dependent stress signaling to DNA replication licensing." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 140-53, 2007 (PubMed).