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Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1) antibody

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Synonyms KLRG1, 2F1-Ag, MAFA, MAFA-L, 2F1, CLEC15A, MAFA-2F1, MAFA-LIKE
Mouse (Murine)
(89), (59), (4)
(54), (30), (30), (9), (6)
Clonality (Clone)
Monoclonal ()
(22), (17), (14), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Flow Cytometry (FACS), Immunoprecipitation (IP)
(82), (29), (26), (21), (3), (2), (2), (2)
Pubmed 5 references available
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Quantity 0.5 mg
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen Activated NK (A-LAK) cells from B6 (H-2b) mice 1
Clone 2F1
Isotype IgG
Characteristics The antibody detects: KLRG1 (Mr 30-38 kDa)
The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1, formerly known as mouse MAFA or 2F1-Ag), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. The 2F1 antibody stains 30-60 % of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.
Purification Purified
Alternative Name KLRG1/MAFA (KLRG1 Antibody Abstract)
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Product cited in: Wilson, Lin, Pack et al.: "Gamma interferon controls mouse polyomavirus infection in vivo." in: Journal of virology, Vol. 85, Issue 19, pp. 10126-34, 2011 (PubMed).

Reiley, Shafiani, Wittmer et al.: "Distinct functions of antigen-specific CD4 T cells during murine Mycobacterium tuberculosis infection." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 45, pp. 19408-13, 2010 (PubMed).

Araki, Turner, Shaffer et al.: "mTOR regulates memory CD8 T-cell differentiation." in: Nature, Vol. 460, Issue 7251, pp. 108-12, 2009 (PubMed).

Background publications Corral, Hanke, Vance et al.: "NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules." in: European journal of immunology, Vol. 30, Issue 3, pp. 920-30, 2000 (PubMed).

Nakamura, Niemi, Fisher et al.: "Mouse Ly-49A interrupts early signaling events in natural killer cell cytotoxicity and functionally associates with the SHP-1 tyrosine phosphatase." in: The Journal of experimental medicine, Vol. 185, Issue 4, pp. 673-84, 1997 (PubMed).

Catalog No. ABIN135349
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