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serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) antibody

Details for Product No. ABIN180930, Supplier: Log in to see
Antigen
  • SERPINB9
  • BB283241
  • CAP-3
  • CAP3
  • PI-9
  • PI9
  • Spi6
  • ovalbumin
Reactivity
Human
85
13
12
1
1
Host
Mouse
43
27
15
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
3
3
3
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
76
41
17
16
14
4
4
2
1
1
1
Supplier
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Immunogen Recombinant PI-9 produced in P. pastoris. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1myeloma cell line. Genename: PI-9
Clone 7D8
Isotype IgG1
Specificity This antibody is specific for SERPINB9/PI-9 (Proteinase Inhibitor 9). Does not react with Mouse and Pig.
Characteristics Synonyms: PI9, Serpin B9, Cytoplasmic antiproteinase 3, CAP-3, CAP3, Proteinase inhibitor 9
Purification Affinity Chromatography on Protein G
Alternative Name SERPINB9 (SERPINB9 Antibody Abstract)
Background PI-9 is a 42 kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virusinfected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.Synonyms: CAP-3, CAP3, Cytoplasmic antiproteinase 3, PI-9, PI9, Proteinase inhibitor 9, Serpin B9
Gene ID 5272
UniProt P50453
Research Area Proteases
Pathways
Application Notes Western Blot: This antibody detects a band of approx. 42 kda under Reducing ConditionsFlow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 1x10^6 cells in 100 μL. Membrane permeabilisation is required for this application (The use of Leucoperm isrecommended for this purpose). Immunohistochemistry on Paraffin Sections: 1/50. This product requires antigen retrieval using heat treatment prior to staining of paraffinsections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil. Also Suitable for use in Indirect Immunofluorescence. (See Ref.2 for more details)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Heutinck, Kassies, Florquin et al.: "SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 27, Issue 7, pp. 2746-54, 2012 (PubMed).

Hirst, Buzza, Bird et al.: "The intracellular granzyme B inhibitor, proteinase inhibitor 9, is up-regulated during accessory cell maturation and effector cell degranulation, and its overexpression enhances CTL potency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 2, pp. 805-15, 2003 (PubMed).

Hirst, Buzza, Sutton et al.: "Perforin-independent expression of granzyme B and proteinase inhibitor 9 in human testis and placenta suggests a role for granzyme B-mediated proteolysis in reproduction." in: Molecular human reproduction, Vol. 7, Issue 12, pp. 1133-42, 2001 (PubMed).

Buzza, Hirst, Bird et al.: "The granzyme B inhibitor, PI-9, is present in endothelial and mesothelial cells, suggesting that it protects bystander cells during immune responses." in: Cellular immunology, Vol. 210, Issue 1, pp. 21-9, 2001 (PubMed).

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