SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (N-Term) antibody

Details for Product No. ABIN182378
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Antigen
Synonyms Dot3, EAP30, VPS22, D11moh34, RGD1310144, D11MOH34, D11Moh34, zgc:101578, vps22, d11moh34
Epitope
N-Term
(7), (5), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Xenopus laevis, Cow (Bovine), Dog (Canine), Zebrafish (Danio rerio), Human, Chicken
(23), (10), (10), (2), (2), (2), (2), (1)
Host
Rabbit
(23), (2), (1)
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(26), (5), (4), (2), (2)
Pubmed 1 reference available
Catalog no. ABIN182378
Quantity 50 µg
Price
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Immunogen Synthetic peptide directed towards the N terminal of human EAP30
Sequence MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQL DMFKTNLEEF
Predicted Reactivity African clawed frog :100 %,   Western clawed frog :100 %,   Bovine :100 %,   Channel catfish :100 %,   blue catfish :100 %,   Dog :100 %,   Chicken :100 %,   Rat :100 %,   Atlantic salmon :100 %,   Mouse :100 %,   Human :100 %,   Japanese pufferfish :90 %,   Zebrafish :90 %,   Florida lancelet :80 %,  
Characteristics This is a rabbit polyclonal antibody against SNF8. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name EAP30
Background ELL encodes an RNA polymerase II transcription factor that undergoes frequent translocation in acute myeloid leukemia (AML). In addition to its elongation activity, ELL contains a novel type of RNA polymerase II interaction domain that is capable of repressing polymerase activity in promoter-specific transcription. EAP30 is a subunit of the ELL complex. EAP30 can interact with ELL and derepress ELL's inhibitory activity in vitro.SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL, MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741], Kamura et al., 2001 [PubMed 11278625]).
Molecular Weight 29 kDa
Gene ID 11267
NCBI Accession NM_007241, NP_009172
UniProt Q96H20
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 258 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (N-Term) antibody anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (N-Term) antibody
anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (N-Term) antibody (2) anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (N-Term) antibody (Image 2)
anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (N-Term) antibody (3) anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (N-Term) antibody (Image 3)
Background publications Schmidt, Miller, Schmidt et al.: "Cloning and characterization of the EAP30 subunit of the ELL complex that confers derepression of transcription by RNA polymerase II." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21981-5, 1999 (PubMed).

Hosts (23), (2), (1)
Reactivities (23), (10), (10), (2), (2), (2), (2), (1)
Applications (26), (5), (4), (2), (2)
Epitopes (7), (5), (2), (2), (1), (1), (1)
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