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General Transcription Factor III (GTF2I) (N-Term) antibody

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Antigen
Synonyms bap-135, bap135, btkap1, diws, gtfii-i, ib291, spin, tfii-i, wbs, wbscr6, 6030441I21Rik, BAP-135, GtfII-I, Spin, TFII-I, WBSCR6, Gtf2ird1, BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, WBS
Epitope
N-Term
(20), (16), (15), (6), (5), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(106), (32), (29), (5), (1), (1)
Host
Rabbit
(90), (16)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(78), (46), (31), (16), (15), (11), (7), (6), (5), (3), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human GTF2I
Blocking Peptide Blocking peptide for this product available: ABIN977774
Sequence ILSPGGSCGP IKVKTEPTED SGISLEMAAV TVKEESEDPD YYQYNIQGSH
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against GTF2I. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name GTF2I (GTF2I Antibody Abstract)
Background GTF2I encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. The exon(s) encoding 5' UTR has not been fully defined, but this gene is known to contain at least 34 exons, and its alternative splicing generates 4 transcript variants.
Molecular Weight 110 kDa
Gene ID 2969
NCBI Accession NP_127493, NM_033000
UniProt Q75M86
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 978 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-General Transcription Factor III (GTF2I) (N-Term) antibody (ABIN182440) WB Suggested Anti-GTF2I Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-General Transcription Factor III (GTF2I) (N-Term) antibody (ABIN182440) anti-General Transcription Factor III (GTF2I) (N-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-General Transcription Factor III (GTF2I) (N-Term) antibody (ABIN182440) anti-General Transcription Factor III (GTF2I) (N-Term) antibody (Image 3)
Western Blotting (WB) image for anti-General Transcription Factor III (GTF2I) (N-Term) antibody (ABIN182440) WB Suggested Anti-GTF2I AntibodyTitration: 2.5 µg/mL
Positive Control: HepG2 W...
Background publications Morris, Mervis, Hobart et al.: "GTF2I hemizygosity implicated in mental retardation in Williams syndrome: genotype-phenotype analysis of five families with deletions in the Williams syndrome region." in: American journal of medical genetics. Part A, Vol. 123A, Issue 1, pp. 45-59, 2003 (PubMed).

Catalog No. ABIN182440
$289.00
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