Transcription Elongation Factor A (SII), 1 (TCEA1) (N-Term) antibody

Details for Product No. ABIN182803
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Antigen
Synonyms gtf2s, sii, tcea, tf2s, TCEA1, S-II, GTF2S, SII, TCEA, TF2S, TFIIS, fj48e12, wu:fj48e12, zgc:63975
Epitope
N-Term
(8), (7), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Human, Chicken
(29), (8), (6), (2), (2), (1), (1), (1)
Host
Rabbit
(26), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(29), (11), (10), (5), (2), (2), (1)
Pubmed 2 references available
Quantity 50 µg
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Catalog No. ABIN182803
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Immunogen Synthetic peptide directed towards the N terminal of human TCEA1
Sequence KSWKKLLDGP STEKDLDEKK KEPAITSQNS PEAREESTSS GNVSNRKDET
Predicted Reactivity Human : 100 %, Guinea pig : 80 %, Mouse : 80 %, Rat : 80 %, Chicken : 70 %, Dog : 70 %, Horse : 70 %, Pig : 70 %
Characteristics This is a rabbit polyclonal antibody against TCEA1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name TCEA1
Background TCEA1 is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. TCEA1 has a role in suppression of transient pausing, which is the most important contribution of TCEA1 to elongation from a stall position. TCEA1 fuse to PLAG1 protein which is overexpressed in epithelial, myoepithelial, and mesenchymal-like tumor cells in tumors.
Molecular Weight 34 kDa
Gene ID 6917
NCBI Accession NM_006756, NP_006747
UniProt P23193
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 301 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Transcription Elongation Factor A (SII), 1 (TCEA1) (N-Term) antibody Immunohistochemistry with Prostate tissue at an antibody concentration of 5µg/ml using anti-TCEA1 antibody (ARP33407_P050)
anti-Transcription Elongation Factor A (SII), 1 (TCEA1) (N-Term) antibody (2) WB Suggested Anti-TCEA1
Antibody Titration: 0.2-1 µg/mL
Positive Control: Transfected 293T
Product cited in: Lazrek, Goffard, Schanen et al.: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006 (PubMed).

Background publications Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Validation Images
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