Enolase 1, (Alpha) (ENO1) (Middle Region) antibody

Details for Product No. ABIN182990
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Antigen
Synonyms Pgh1, pENO1, Eno1, ENO1L1, MPB1, NNE, PPH, eno1, eno1b, eno1l1, mbp-1, mpb1, nne, pph, Nne, 0610008I15, AL022784, Eno-1, MBP-1, wu:fi32b03, wu:fk58e02, zgc:73152
Epitope
Middle Region
(11), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Pig (Porcine), Human, Chicken
(90), (35), (23), (13), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(74), (27), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(94), (59), (23), (15), (12), (10), (5), (5), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN182990
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human ENO1
Sequence VAASEFFRSG KYDLDFKSPD DPSRYISPDQ LADLYKSFIK DYPVVSIEDP
Predicted Reactivity Chicken : 100 %, Human : 100 %, Pig : 100 %, Dog : 90 %, Guinea pig : 90 %, Mouse : 90 %, Rabbit : 90 %, Rat : 90 %, Zebrafish : 90 %, African clawed frog : 80 %, Horse : 70 %
Characteristics This is a rabbit polyclonal antibody against ENO1. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name ENO1
Background ENO1 encodes one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Molecular Weight 47 kDa
Gene ID 2023
NCBI Accession NP_001419, NM_001428
UniProt Q53HR3
Research Area Cancer, Transcription Factors, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 434 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody
anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody (2) Rabbit Anti-ENO1 Antibody ,Paraffin Embedded Tissue: Human placenta cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 µg/mL
Magnification:.00X
anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody (3) Human kidney
anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody (4) WB Suggested Anti-ENO1
Antibody Titration: 0.03 µg/mL ELISA Titer: 1:1562500
Positive Control: Human Small Intestine
Background publications Lee, Chung, Kim et al.: "Human alpha-enolase from endothelial cells as a target antigen of anti-endothelial cell antibody in Behçet's disease." in: Arthritis and rheumatism, Vol. 48, Issue 7, pp. 2025-35, 2003 (PubMed).

Validation Images
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