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Enolase 1, (Alpha) (ENO1) (Middle Region) antibody

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Synonyms Pgh1, pENO1, Eno1, ENO1L1, MPB1, NNE, PPH, eno1, eno1b, eno1l1, mbp-1, mpb1, nne, pph, Nne, 0610008I15, AL022784, Eno-1, MBP-1, wu:fi32b03, wu:fk58e02, zgc:73152
Middle Region
(11), (10), (9), (7), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(122), (39), (19), (3), (2), (2), (2), (1), (1), (1), (1), (1)
(99), (42), (4)
(11), (8), (8), (1), (1), (1), (1)
Immunohistochemistry (IHC), Western Blotting (WB)
(133), (92), (31), (26), (23), (7), (7), (6), (4), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Quantity 50 μg
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen Synthetic peptide directed towards the middle region of human ENO1
Blocking Peptide Blocking peptide for this product available: ABIN976344
Predicted Reactivity Chicken : 100 %, Human : 100 %, Pig : 100 %, Dog : 90 %, Guinea pig : 90 %, Mouse : 90 %, Rabbit : 90 %, Rat : 90 %, Zebrafish : 90 %, African clawed frog : 80 %, Horse : 70 %
Characteristics This is a rabbit polyclonal antibody against ENO1. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name ENO1 (ENO1 Antibody Abstract)
Background ENO1 encodes one of three enolase isoenzymes found in mammals, it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Molecular Weight 47 kDa
Gene ID 2023
NCBI Accession NP_001419, NM_001428
UniProt Q53HR3
Research Area Cancer, Transcription Factors, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 434 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Immunohistochemistry (IHC) image for anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody (ABIN182990) anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody (ABIN182990) Rabbit Anti-ENO1 Antibody ,Paraffin Embedded Tissue: Human placenta cell
Western Blotting (WB) image for anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody (ABIN182990) WB Suggested Anti-ENO1
Antibody Titration: 0.03 µg/mL ELISA Titer: 1:1562500 <...
Immunohistochemistry (IHC) image for anti-Enolase 1, (Alpha) (ENO1) (Middle Region) antibody (ABIN182990) Human kidney
Product cited in: Kitahashi, Yoshimoto, Imai: "Novel immunohistochemical marker, integrin α(V)β(3), for BOP-induced early lesions in hamster pancreatic ductal carcinogenesis." in: Oncology letters, Vol. 2, Issue 2, pp. 229-234, 2012 (PubMed).

Deng, Lam, Meyer et al.: "Frontal-subcortical protein expression following prenatal exposure to maternal inflammation." in: PLoS ONE, Vol. 6, Issue 2, pp. e16638, 2011 (PubMed).

Sørensen, Horsman, Vorum et al.: "Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 92, Issue 3, pp. 443-9, 2009 (PubMed).

Boraldi, Annovi, Carraro et al.: "Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A proteomic approach." in: Biochimica et biophysica acta, Vol. 1774, Issue 11, pp. 1402-13, 2007 (PubMed).

Background publications Lee, Chung, Kim et al.: "Human alpha-enolase from endothelial cells as a target antigen of anti-endothelial cell antibody in Behçet's disease." in: Arthritis and rheumatism, Vol. 48, Issue 7, pp. 2025-35, 2003 (PubMed).

Catalog No. ABIN182990
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