RRN3 RNA Polymerase I Transcription Factor Homolog (S. Cerevisiae) (RRN3) (Middle Region) antibody

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Antigen
Epitope
Middle Region
(15), (10), (10), (9), (6), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(52), (4), (4), (4), (3), (3), (2)
Host
Rabbit
(47), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(50), (36), (12), (2), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the middle region of human RRN3
Sequence VDGKVDNGKT KDLYRDLINI FDKLLLPTHA SCHVQFFMFY LCSFKLGFAE
Predicted Reactivity Human : 100 %, Dog : 90 %, Chicken : 90 %, Bovine : 80 %, Guinea pig : 80 %, Horse : 80 %, Mouse : 80 %, Rabbit : 80 %, Rat : 80 %
Characteristics This is a rabbit polyclonal antibody against RRN3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name RRN3 (RRN3 Antibody Abstract)
Background In mammals, growth-dependent regulation of RNA polymerase I (Pol I) transcription is mediated by RRN3, an essential initiation factor. It interacts with Pol I in the absence of template DNA, augments Pol I transcription in vivo and rescues transcription in extracts from growth-arrested cells in vitro.
Molecular Weight 74 kDa
Gene ID 54700
NCBI Accession NM_018427, NP_060897
UniProt Q9NYV6
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 650 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-RRN3 RNA Polymerase I Transcription Factor Homolog (S. Cerevisiae) (RRN3) (Middle Region) antibody (ABIN183013) WB Suggested Anti-RRN3 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:...
Background publications Miller, Panov, Friedrich et al.: "hRRN3 is essential in the SL1-mediated recruitment of RNA Polymerase I to rRNA gene promoters." in: The EMBO journal, Vol. 20, Issue 6, pp. 1373-82, 2001 (PubMed).