CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (N-Term) antibody

Details for Product No. ABIN183293
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Antigen
Synonyms CTCFL, BORIS, CT27, CTCF-T, HMGB1L1, dJ579F20.2, Boris, OTTMUSG00000016680
Epitope
N-Term
(12), (3), (3), (1), (1)
Reactivity
Human
(26), (19), (16), (12), (12), (12), (1)
Host
Rabbit
(29), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(22), (10), (5), (5), (3), (3), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN183293
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human CTCFL
Sequence RSDEIVLTVS NSNVEEQEDQ PTAGQADAEK AKSTKNQRKT KGAKGTFHCD
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against CTCFL. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CTCFL
Background CTCFL is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes. CTCFL is normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation.
Molecular Weight 76 kDa
Gene ID 140690
NCBI Accession NP_542185, NM_080618
UniProt Q8NI51
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 663 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (N-Term) antibody WB Suggested Anti-CTCFL Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate
anti-CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (N-Term) antibody (2) WB Suggested Anti-CTCFL
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500
Positive Control: Jurkat cell lysate
CTCFL is supported by BioGPS gene expression data to be expressed in Jurkat
Background publications Hong, Kang, Abdullaev et al.: "Reciprocal binding of CTCF and BORIS to the NY-ESO-1 promoter coincides with derepression of this cancer-testis gene in lung cancer cells." in: Cancer research, Vol. 65, Issue 17, pp. 7763-74, 2005 (PubMed).

Validation Images
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