Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (C-Term) antibody

Details for Product No. ABIN184580
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Antigen
Synonyms A630013F22Rik, AW552122, EPI, LACI, TFI, TFPI1
Epitope
C-Term
(12), (6), (6), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(89), (26), (21), (12), (12), (3), (1), (1), (1), (1)
Host
Goat
(67), (28), (8), (5), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(69), (61), (20), (10), (10), (9), (8), (8), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184580
374.27 $
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Immunogen Peptide with sequence C-VKIAYEEIFVKNM, from the C Terminus of the protein sequence according to NP_006278.1.
Specificity This antibody is expected to recognize isoform a (NP_006278.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Tissue Factor Pathway Inhibitor
Background Synonyms: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor), TFPI, EPI, LACI, TFI, TFPI1, OTTHUMP00000163472, OTTHUMP00000205431, anti-convertin, extrinsic pathway inhibitor, lipoprotein-associated coagulation inhibitor, tissue factor pathway inhibitor
Gene ID 7035
NCBI Accession NP_006278
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 50 kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0 kDa according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38 kDa to 45 kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (C-Term) antibody ABIN184580 staining (0.3 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Wun, Kretzmer, Girard et al.: "Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains." in: The Journal of biological chemistry, Vol. 263, Issue 13, pp. 6001-4, 1988 (PubMed).

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