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SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) (C-Term) antibody

Details for Product No. ABIN184621, Supplier: Login to see New
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Synonyms im:5777505, wu:fa08f03, wu:fb13e12, MGC79086, sh3glb1, wu:fc55h11, wu:fj98d09, AA409932, AI314629, AU015566, Bif-1, mKIAA0491, PPP1R70, dJ612B15.2
(24), (12), (7), (7), (5), (4), (4), (4), (2), (2), (1), (1)
(100), (34), (29), (2), (1), (1), (1), (1), (1)
(78), (16), (7), (1)
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(74), (41), (38), (23), (11), (9), (6), (5), (3), (2)
Pubmed 1 reference available
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Quantity 100 μg
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Availability Available
Immunogen Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1.
Blocking Peptide Blocking peptide for this product available: ABIN369652
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BIF-1 / SH3GLB1 (SH3GLB1 Antibody Abstract)
Background Synonyms: SH3GLB1, Bif-1, SH3-domain GRB2-like endophilin B1, CGI-61, KIAA0491, endophilin B1, Bax-interacting factor 1, SH3-containing protein SH3GLB1, SH3-domain, GRB2-like, endophilin B1, SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1, dJ612B15.2
Gene ID 51100, 54673
NCBI Accession NP_057093
Research Area Autophagy
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38 kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8 kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 µg/mL. A minor band of unknown identity was also consistently observed at 50 kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) (C-Term) antibody (ABIN184621) ABIN184621 staining (0.3µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per ...
Background publications Takahashi, Karbowski, Yamaguchi et al.: "Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9369-82, 2005 (PubMed).

Catalog No. ABIN184621
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