SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) (C-Term) antibody

Details for Product No. ABIN184621
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Antigen
Synonyms im:5777505, wu:fa08f03, wu:fb13e12, MGC79086, sh3glb1, wu:fc55h11, wu:fj98d09, AA409932, AI314629, AU015566, Bif-1, mKIAA0491, PPP1R70, dJ612B15.2
Epitope
C-Term
(15), (7), (7), (6), (3), (3), (3), (1), (1), (1), (1)
Reactivity
Human
(48), (13), (8), (2), (1)
Host
Goat
(37), (9), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(43), (23), (16), (11), (7), (6), (4), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184621
374.27 $
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Immunogen Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BIF-1 / SH3GLB1
Background Synonyms: SH3GLB1, Bif-1, SH3-domain GRB2-like endophilin B1, CGI-61, KIAA0491, endophilin B1, Bax-interacting factor 1, SH3-containing protein SH3GLB1, SH3-domain, GRB2-like, endophilin B1, SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1, dJ612B15.2
Gene ID 51100, 54673
NCBI Accession NP_057093
Research Area Autophagy
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38 kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8 kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 µg/mL. A minor band of unknown identity was also consistently observed at 50 kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) (C-Term) antibody ABIN184621 staining (0.3µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Takahashi, Karbowski, Yamaguchi et al.: "Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9369-82, 2005 (PubMed).

Validation Images
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