Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibody

Details for Product No. ABIN184710
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Antigen
Synonyms Dkk-4, DKK-4, RGD1563172
Epitope
C-Term
(23), (10), (8), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(43), (7), (2)
Host
Goat
(39), (6), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(46), (27), (13), (8), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184710
374.27 $
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Immunogen Peptide with sequence RQHARLRVCQKIEKL, from the C Terminus of the protein sequence according to NP_055235.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DKK4
Background Synonyms: DKK4, dickkopf homolog 4 (Xenopus laevis), DKK-4, hdkk-4, Dickkopf gene 4, dickkopf (Xenopus laevis) homolog 4, MGC129562, MGC129563, dickkopf homolog 4
Gene ID 27121
NCBI Accession NP_055235
Research Area Stem Cells, Wnt
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30-35 kDa band seen in Jurkat lysate. Recommended for use at 1-3 µg/mL. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32 kDa species of DKK4.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibody ABIN184710 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).

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