Phosphatidylethanolamine Binding Protein 1 (PEBP1) (C-Term) antibody

Details for Product No. ABIN184729
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Antigen
Synonyms
BcDNA:LP12095, CG18594, Dmel\\CG18594, PEBP1, T2, pbp, hcnp, pebp, rkip, HCNP, HCNPpp, PBP, PEBP, PEBP-1, RKIP, Pbp, Rkip, Pbp1, Pbpr, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MD ... show more
BcDNA:LP12095, CG18594, Dmel\\CG18594, PEBP1, T2, pbp, hcnp, pebp, rkip, HCNP, HCNPpp, PBP, PEBP, PEBP-1, RKIP, Pbp, Rkip, Pbp1, Pbpr, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1 show less
Epitope
C-Term
(19), (13), (13), (8), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(109), (47), (47), (24), (19), (16), (13), (1), (1), (1), (1), (1)
Host
Goat
(91), (17), (14), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(96), (67), (27), (24), (19), (2)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN184729
374.27 $
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Immunogen Peptide with sequence C-DYVPKLYEQLSGK, from the C Terminus of the protein sequence according to NP_002558.1.
Cross-Reactivity Mouse (Murine), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PEBP1
Background Synonyms: PEBP1, phosphatidylethanolamine binding protein 1, HCNP, PBP, PEBP, RKIP, prostatic binding protein, RAF kinase inhibitor protein, Raf kinase inhibitory protein, hippocampal cholinergic neurostimulating peptide
Gene ID 5037, 23980
NCBI Accession NP_002558
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 22 kDa band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 21.1 kDa according to NP_002558.1). Recommended concentration: 0.01-0.03 µg/mL. A customer has reported the 22 kDa in Rat Hippocampus using this antibody.
IHC: In paraffin embedded Human Brain (Cortex) shows staining near sectect nuclei. Recommended concentration: 1-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (C-Term) antibody ABIN184729 staining (0.01µg/ml) of A549 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product cited in: Weitzdörfer, Höger, Shim et al.: "Changes of hippocampal signaling protein levels during postnatal brain development in the rat." in: Hippocampus, Vol. 18, Issue 8, pp. 807-13, 2008 (PubMed).

General Yeung, Seitz, Li et al.: "Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP." in: Nature, Vol. 401, Issue 6749, pp. 173-7, 1999 (PubMed).

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