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DAP3 antibody (Death-Associated Protein 3) (C-Term)

Details for Product anti-DAP3 Antibody No. ABIN184743, Supplier: Log in to see
Antigen
  • DAP-3
  • MRP-S29
  • MRPS29
  • bMRP-10
  • 4921514D13Rik
  • BC058433
  • DAP3
  • Prpl8
  • Sapap3
Epitope
C-Term
16
14
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
86
34
23
1
1
Host
Goat
50
23
16
Clonality
Polyclonal
Conjugate
This DAP3 antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
71
30
14
11
9
3
3
1
Supplier
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Immunogen Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1, NP_001186779.1, NP_001186780.1.
Blocking Peptide Blocking peptide for this product available: ABIN370267
Specificity This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DAP3 (DAP3 Antibody Abstract)
Background Synonyms: DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29
Gene ID 7818
NCBI Accession NP_001186779, NP_387506, NP_001186780
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HeLa (calculated MW of 45.6 kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37 kDa). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-DAP3 antibody (Death-Associated Protein 3) (C-Term) (ABIN184743) ABIN184743 staining (3 µg/mL) of HeLa lysate (RIPA buffer, 30 µg total protein per la...
 image for anti-DAP3 antibody (Death-Associated Protein 3) (C-Term) (ABIN184743) ABIN184743 (1 µg/mL) staining of HeLa lysate (control in left lane and after si-RNA-m...
 image for anti-DAP3 antibody (Death-Associated Protein 3) (C-Term) (ABIN184743) HEK293 overexpressing DAP3 (RC223182) and probed with ABIN184743 (mock transfection i...
Background publications Kissil, Deiss, Bayewitch et al.: "Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death." in: The Journal of biological chemistry, Vol. 270, Issue 46, pp. 27932-6, 1996 (PubMed).