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Details for Product No. ABIN184743

Death-Associated Protein 3 (DAP3) (C-Term) antibody

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Antigen
Synonyms DAP-3, 4921514D13Rik, MRP-S29, MRPS29, bMRP-10
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN184743
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1, NP_001186779.1, NP_001186780.1.
Specificity This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DAP3
Background Synonyms: DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29
Gene ID 7818
NCBI Accession NP_387506.1, NP_001186779.1, NP_001186780.1
Research Area Apoptosis/Necrosis, DNA/RNA
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HeLa (calculated MW of 45.6 kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37 kDa). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Kissil, Deiss, Bayewitch et al.: "Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death." in: The Journal of biological chemistry, Vol. 270, Issue 46, pp. 27932-6, 1996 (PubMed).

Alternatives for antigen "Death-Associated Protein 3 (DAP3)", type "Antibodies"
Hosts (28), (10), (6)
Reactivities (41), (17), (12)
Applications (32), (13), (9), (5), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (3), (2), (2), (1)
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