anti-DAP3 antibody (Death-Associated Protein 3) (C-Term)

Details for Product anti-DAP3 Antibody No. ABIN184743
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Synonyms DAP-3, MRP-S29, MRPS29, bMRP-10, 4921514D13Rik, BC058433, DAP3, Prpl8, Sapap3
(17), (14), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(84), (28), (19), (1), (1)
(47), (23), (17)
This DAP3 antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(71), (32), (12), (10), (9), (5), (3), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Immunogen Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1, NP_001186779.1, NP_001186780.1.
Specificity This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DAP3 (DAP3 Antibody Abstract)
Background Synonyms: DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29
Gene ID 7818
NCBI Accession NP_001186779, NP_387506, NP_001186780
Research Area Apoptosis/Necrosis, DNA/RNA
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HeLa (calculated MW of 45.6 kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37 kDa). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-DAP3 antibody (Death-Associated Protein 3) (C-Term) (ABIN184743) ABIN184743 staining (3 µg/mL) of HeLa lysate (RIPA buffer, 30 µg total protein per la...
Image no. 2 for anti-DAP3 antibody (Death-Associated Protein 3) (C-Term) (ABIN184743) ABIN184743 (1 µg/mL) staining of HeLa lysate (control in left lane and after si-RNA-m...
Image no. 3 for anti-DAP3 antibody (Death-Associated Protein 3) (C-Term) (ABIN184743) HEK293 overexpressing DAP3 (RC223182) and probed with ABIN184743 (mock transfection i...
Background publications Kissil, Deiss, Bayewitch et al.: "Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death." in: The Journal of biological chemistry, Vol. 270, Issue 46, pp. 27932-6, 1996 (PubMed).

Validation Images
Catalog No. ABIN184743
$ 325.97
Plus shipping costs $45.00
100 μg
$ 325.97

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