Death-Associated Protein 3 (DAP3) (C-Term) antibody

Details for Product No. ABIN184743
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Antigen
Synonyms DAP-3, 4921514D13Rik, MRP-S29, MRPS29, bMRP-10
Epitope
C-Term
(5), (2), (1), (1), (1), (1)
Reactivity
Human
(40), (18), (13)
Host
Goat
(32), (6), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(31), (14), (9), (5), (4), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN184743
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1, NP_001186779.1, NP_001186780.1.
Specificity This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DAP3
Background Synonyms: DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29
Gene ID 7818
NCBI Accession NP_387506.1, NP_001186779.1, NP_001186780.1
Research Area Apoptosis/Necrosis, DNA/RNA
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in lysates of cell line HeLa (calculated MW of 45.6 kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37 kDa). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Death-Associated Protein 3 (DAP3) (C-Term) antibody ABIN184743 staining (3 µg/mL) of HeLa lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Death-Associated Protein 3 (DAP3) (C-Term) antibody (2) ABIN184743 (1 µg/mL) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35 µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was detemined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Death-Associated Protein 3 (DAP3) (C-Term) antibody (3) HEK293 overexpressing DAP3 (RC223182) and probed with ABIN184743 (mock transfection in first lane), tested by Origene.
General Kissil, Deiss, Bayewitch et al.: "Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death." in: The Journal of biological chemistry, Vol. 270, Issue 46, pp. 27932-6, 1996 (PubMed).

Hosts (32), (6), (5)
Reactivities (40), (18), (13)
Applications (31), (14), (9), (5), (4), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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