Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) (C-Term) antibody

Details for Product No. ABIN184798
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Antigen
Synonyms GB11574, rse1, sap130, sf3b130, staf130, sf3b3, 1810061H24Rik, 5730409A01Rik, AA409318, D8Ertd633e, RSE1, SAP130, mKIAA0017, ik:tdsubc_2b2, wu:fb81f05, xx:tdsubc_2b2, zgc:55440, SF3b130, STAF130
Epitope
C-Term
(12), (11), (8), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(43), (17), (7), (3), (2), (1), (1)
Host
Goat
(38), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(41), (24), (7), (5), (4), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN184798
374.27 $
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Immunogen Peptide with sequence C-VSKKLEDIRTRYAF, from the C Terminus of the protein sequence according to NP_036558.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SAP130 / SF3B3
Background Synonyms: SF3B3, SAP130, splicing factor 3b, subunit 3, 130kDa, RSE1, SF3B130, KIAA0017, STAF130, pre-mRNA splicing factor SF3b, 130 kDa subunit, spliceosome-associated protein 130, splicing factor 3b, subunit 3, SF3b130
Gene ID 23450, 101943
NCBI Accession NP_036558
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 150 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136 kDa according to NP_036558.3). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) (C-Term) antibody ABIN184798 staining (0.1µg/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Das, Xia, Palandjian et al.: "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 6796-802, 2000 (PubMed).

Validation Images
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