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Golgi Membrane Protein 1 (GOLM1) (C-Term) antibody

Details for Product No. ABIN184847, Supplier: Log in to see
Antigen
  • GOLM1
  • GOLPH2
  • C9orf155
  • GP73
  • PSEC0257
  • bA379P1.3
  • 2310001L02Rik
  • AW125446
  • D030064E01Rik
  • Golph2
  • golph2
  • gp73
  • 0610011A07Rik
  • 2410043M02Rik
  • 5730520M13Rik
  • 9430094F20Rik
  • AW552058
  • GRASP55
  • GRS2
  • p59
Epitope
C-Term
16
9
7
7
6
5
5
4
4
4
2
2
2
2
2
1
1
1
Reactivity
Human
153
20
14
4
2
2
1
1
1
Host
Goat
92
51
16
Clonality
Polyclonal
Conjugate
Un-conjugated
12
10
6
2
2
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
107
89
65
38
37
13
7
4
4
4
3
2
2
1
Supplier
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Immunogen Peptide with sequence C-NLLDQREKRNHTL, from the C Terminus of the protein sequence according to NP_057632.2, NP_808800.1.
Blocking Peptide Blocking peptide for this product available: ABIN369482
Specificity Both variants (NP_057632.2, NP_808800.1) encode the same isoform
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GOLPH2 (GOLM1 Antibody Abstract)
Background Synonyms: GOLM1, GOLPH2, golgi phosphoprotein 2, GP73, golgi membrane protein GP73, golgi membrane protein 1, RP11-379P1.3, C9orf155, FLJ22634, FLJ23608, PSEC0257, bA379P1.3, golgi protein, 73-kD
Gene ID 51280
NCBI Accession NP_057632, NP_808800
Research Area Signaling
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing full-length Human GOLM1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.2-1 µg/mL.
IHC: In paraffin embedded Human Prostate shows luminal staining of secretory cells in the glands. Similar results were obtained in Human Uterus and Small intestine. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Kladney, Bulla, Guo et al.: "GP73, a novel Golgi-localized protein upregulated by viral infection." in: Gene, Vol. 249, Issue 1-2, pp. 53-65, 2000 (PubMed).