Golgi Membrane Protein 1 (GOLM1) (C-Term) antibody

Details for Product No. ABIN184847
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Antigen
Synonyms
GOLM1, GOLPH2, C9orf155, GP73, PSEC0257, bA379P1.3, 2310001L02Rik, AW125446, D030064E01Rik, Golph2, golph2, gp73, 0610011A07Rik, 2410043M02Rik, 5730520M13Rik, 9430094F20Rik, AW552058, GRASP55, GRS2, p ... show more
GOLM1, GOLPH2, C9orf155, GP73, PSEC0257, bA379P1.3, 2310001L02Rik, AW125446, D030064E01Rik, Golph2, golph2, gp73, 0610011A07Rik, 2410043M02Rik, 5730520M13Rik, 9430094F20Rik, AW552058, GRASP55, GRS2, p59 show less
Epitope
C-Term
(4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(88), (34), (34), (25), (13), (13), (12), (3), (1)
Host
Goat
(61), (23), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(67), (37), (27), (25), (20), (19), (6), (3), (2), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184847
374.27 $
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Immunogen Peptide with sequence C-NLLDQREKRNHTL, from the C Terminus of the protein sequence according to NP_057632.2, NP_808800.1.
Specificity Both variants (NP_057632.2, NP_808800.1) encode the same isoform
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GOLPH2
Background Synonyms: GOLM1, GOLPH2, golgi phosphoprotein 2, GP73, golgi membrane protein GP73, golgi membrane protein 1, RP11-379P1.3, C9orf155, FLJ22634, FLJ23608, PSEC0257, bA379P1.3, golgi protein, 73-kD
Gene ID 51280
NCBI Accession NP_057632, NP_808800
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing full-length Human GOLM1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.2-1 µg/mL.
IHC: In paraffin embedded Human Prostate shows luminal staining of secretory cells in the glands. Similar results were obtained in Human Uterus and Small intestine. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Kladney, Bulla, Guo et al.: "GP73, a novel Golgi-localized protein upregulated by viral infection." in: Gene, Vol. 249, Issue 1-2, pp. 53-65, 2000 (PubMed).

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