Heat Shock 22kDa Protein 8 (HSPB8) (C-Term) antibody

Details for Product No. ABIN184852
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Antigen
Synonyms MGC64408, fc09c11, wu:fc04b04, wu:fc09c11, zgc:64202, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, AU018630, AW413033, Cryac, D5Ucla4, H11K, HSP20-like, Hsp22
Epitope
C-Term
(7), (6), (5), (3), (3), (2), (1), (1), (1)
Reactivity
Human
(86), (26), (23), (13), (13), (12)
Host
Goat
(41), (38), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(72), (46), (37), (25), (11), (10), (8), (5), (4), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184852
374.27 $
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Immunogen Peptide with sequence NELPQDSQEVTCT, from the C Terminus of the protein sequence according to NP_055180.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name H11 / HSP22
Background Synonyms: HSPB8, heat shock 22kDa protein 8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, Charcot-Marie-Tooth disease, spinal, heat shock 27kDa protein 8, hereditary motor neuropathy, distal, protein kinase H11, small stress protein-like protein HSP22, spinal muscular atrophy, distal, adult, autosomal dominant, E2-induced gene 1, heat shock protein beta-8
Gene ID 26353
NCBI Accession NP_055180
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 20-25 kDa band seen in human skeletal muscle lysate [Predicted MW of approx. 22 kDa according to NP_055180]. Recommended for use at 0.5-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Heat Shock 22kDa Protein 8 (HSPB8) (C-Term) antibody ABIN184852 staining (1µg/ml) of human muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Benndorf, Sun, Gilmont et al.: "HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27)." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 26753-61, 2001 (PubMed).

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