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N-Myc Downstream Regulated 1 (NDRG1) (C-Term) antibody

Details for Product No. ABIN184872, Supplier: Login to see
Antigen
  • NDRG1
  • CAP43
  • CMT4D
  • DRG-1
  • DRG1
  • GC4
  • HMSNL
  • NDR1
  • NMSL
  • PROXY1
  • RIT42
  • RTP
  • TARG1
  • TDD5
  • cap43
  • cmt4d
  • drg1
  • gc4
  • hmsnl
  • ndr1
  • ndrg1
  • nmsl
  • proxy1
  • rit42
  • rtp
  • targ1
  • tdd5
  • xndrg1
  • Ndr1
  • Ndrl
  • cb775
  • wu:fb60h02
  • zgc:63944
  • NON-RACE SPECIFIC DISEASE RESISTANCE PROTEIN
  • non race-specific disease resistance 1
Epitope
C-Term
27
22
14
6
5
5
4
4
3
2
1
1
1
1
1
1
1
1
Reactivity
Human
112
41
36
10
4
2
2
2
1
1
1
1
1
Host
Goat
87
22
7
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
3
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
98
47
44
25
15
10
6
5
5
3
1
Supplier
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Immunogen Peptide with sequence GNSAGPKSMEVSC, from the C Terminus of the protein sequence according to NP_006087.2, NP_001128714.1.
Blocking Peptide Blocking peptide for this product available: ABIN370161
Specificity The variants (NP_006087.2, NP_001128714.1) encode the same protein
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NDRG1 (NDRG1 Antibody Abstract)
Background Synonyms: NDRG1, N-myc downstream regulated gene 1, CAP43,, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, N-myc downstream regulated gene 1 protein, differentiation-related gene 1 protein, nickel-specific induction protein Cap43, protein regulated by oxygen-1, reducing agents and tunicamycin-responsive protein, tunicamycin-responsive protein
Molecular Weight 42.8kDa
Gene ID 10397, 17990
NCBI Accession NP_001128714, NP_006087
Research Area Cancer, Angiogenesis, Transcription Factors
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48 kDa band observed in lysates of Human Brain, Kidney and Testis (calculated MW of 42.8 kDa according to NP_006087.2). Recommended concentration: 0.1-0.3 µg/mL. A minor band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cyrtoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-N-Myc Downstream Regulated 1 (NDRG1) (C-Term) antibody (ABIN184872) ABIN184872 staining (0.1µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protei...
Background publications Zhou, Salnikow, Costa: "Cap43, a novel gene specifically induced by Ni2+ compounds." in: Cancer research, Vol. 58, Issue 10, pp. 2182-9, 1998 (PubMed).