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Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody

Details for Product No. ABIN184894, Supplier: Log in to see
Antigen
  • UBC9
  • T13J8.70
  • T13J8_70
  • UBIQUITIN-PROTEIN LIGASE
  • ubiquitin conjugating enzyme 9
  • C358B7.1
  • P18
  • 5830467E05Rik
  • F830028O17Rik
  • Ubce2i
  • Ubce9
  • UbcE2A
  • ubc9
  • ube2ia
  • zUbc9
  • ubce9
Epitope
N-Term
35
5
3
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
106
32
25
9
6
3
3
2
2
2
1
1
Host
Goat
70
20
17
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
4
4
4
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
96
59
49
10
10
9
6
5
5
2
1
1
Supplier
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Immunogen Peptide with sequence SGIALSRLAQERK-C, from the N Terminus of the protein sequence according to NP_003336.1, NP_919235.1, NP_919236.1, NP_919237.1.
Blocking Peptide Blocking peptide for this product available: ABIN370449
Specificity This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name UBE2I (UBE2I Antibody Abstract)
Background Synonyms: UBE2I, ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), C358B7.1, P18, UBC9, SUMO-1-protein ligase, ubiquitin carrier protein, ubiquitin conjugating enzyme 9, ubiquitin-conjugating enzyme E2I, ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-conjugating enzyme UbcE2A, ubiquitin-like protein SUMO-1 conjugating enzyme, ubiquitin-protein ligase E2I, OTTHUMP00000198126, OTTHUMP00000198127
Gene ID 7329, 22196, 25573
NCBI Accession NP_003336, NP_919237, NP_919235, NP_919236
Pathways
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 17 kDa band observed in Human Kidney, Lung and Testis lysates and in Mouse and Rat Heart, Spleen and Testis lysates (calculated MW of 18.0 kDa according to Human NP_003336.1). Recommended concentration: 0.1-0.3 µg/mL
IHC: In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration: 1 µg/mL. Immunocytochemsitry: An anonymous customer found positive results in Mouse oocytes.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (ABIN184894) ABIN184894 (0.1 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer)...
Immunohistochemistry (IHC) image for anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (ABIN184894) ABIN184894 (0.3 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antige...
Background publications Wang, Qiu, Seufert et al.: "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24811-6, 1996 (PubMed).