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Ubiquitin-Conjugating Enzyme E2O (UBE2O) (C-Term) antibody

Details for Product No. ABIN184896, Supplier: Log in to see
Antigen
  • e2-230k
  • E2-230K
  • 9630022H21
  • B230113M03Rik
  • mKIAA1734
  • RGD1310297
Epitope
C-Term
7
2
2
1
1
1
1
1
1
Reactivity
Human
22
21
20
4
3
3
1
1
1
Host
Goat
24
9
2
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
12
11
11
7
3
1
1
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Immunogen Peptide with sequence ALLEAGMPECTEDK, from the C Terminus of the protein sequence according to NP_071349.3.
Blocking Peptide Blocking peptide for this product available: ABIN368751
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name E2-230K / UBE2O (UBE2O Antibody Abstract)
Background Synonyms: UBE2O, ubiquitin-conjugating enzyme E2O, E2-230K, FLJ12878, KIAA1734
Gene ID 63893, 217342
NCBI Accession NP_071349
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
IHC: In paraffin embedded Human Heart shows textured staining in some myocardial fibres. Recommended concentration: 4-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Yokota, Nagai, Harada et al.: "Identification, tissue expression, and chromosomal position of a novel gene encoding human ubiquitin-conjugating enzyme E2-230k." in: Gene, Vol. 267, Issue 1, pp. 95-100, 2001 (PubMed).