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Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody

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Antigen
Synonyms UBE2T, wu:fj86h11, zgc:153687, HR6B, PIG50, 2700084L22Rik, C80607, RGD1310816, hspc150
Epitope
C-Term
(30), (15), (6), (6), (5), (3), (2), (2), (1), (1)
Reactivity
Human
(67), (12), (6)
Host
Goat
(43), (15), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(61), (39), (9), (8), (2), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-QLVGIEKKFHPDV, from the C Terminus of the protein sequence according to NP_054895.1.
Blocking Peptide Blocking peptide for this product available: ABIN370326
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name HSPC150 / UBE2T (Ube2t Antibody Abstract)
Background Synonyms: UBE2T, HSPC150, PIG50, HSPC150 protein similar to ubiquitin-conjugating enzyme, ubiquitin conjugating enzyme, ubiquitin-conjugating enzyme E2T (putative), ubiquitin-conjugating enzyme E2T
Gene ID 29089
NCBI Accession NP_054895
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 22 kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5 kDa according to NP_054895.1). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Machida, Machida, Chen et al.: "UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation." in: Molecular cell, Vol. 23, Issue 4, pp. 589-96, 2006 (PubMed).

Catalog No. ABIN184897
$318.20
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