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Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody

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Synonyms UBE2T, wu:fj86h11, zgc:153687, HR6B, PIG50, 2700084L22Rik, C80607, RGD1310816, hspc150
(30), (15), (6), (6), (5), (3), (2), (2), (1), (1)
(67), (12), (6)
(43), (15), (9)
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(61), (39), (9), (8), (2), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-QLVGIEKKFHPDV, from the C Terminus of the protein sequence according to NP_054895.1.
Blocking Peptide Blocking peptide for this product available: ABIN370326
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name HSPC150 / UBE2T (Ube2t Antibody Abstract)
Background Synonyms: UBE2T, HSPC150, PIG50, HSPC150 protein similar to ubiquitin-conjugating enzyme, ubiquitin conjugating enzyme, ubiquitin-conjugating enzyme E2T (putative), ubiquitin-conjugating enzyme E2T
Gene ID 29089
NCBI Accession NP_054895
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 22 kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5 kDa according to NP_054895.1). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Machida, Machida, Chen et al.: "UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation." in: Molecular cell, Vol. 23, Issue 4, pp. 589-96, 2006 (PubMed).

Catalog No. ABIN184897
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