Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F) (C-Term) antibody

Details for Product No. ABIN184898
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Antigen
Synonyms AI851109, 2510010F15Rik, NCE2, RGD1307608, zgc:77005
Epitope
C-Term
(5), (4), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
(30), (20), (19), (17), (16), (13), (12), (1)
Host
Goat
(24), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(21), (13), (10), (6), (2), (1)
Pubmed 1 reference available
Catalog no. ABIN184898
Quantity 100 µg
Price
261.61 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-RNKVDDYIKRYAR, from the C Terminus of the protein sequence according to NP_542409.1.
Specificity This antibody is expected to recognise isoform 1 (NP_542409.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name UBE2F/NEDD8-conjugating enzyme
Background Synonyms: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme
Gene ID 140739
NCBI Accession NP_542409.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Podust, Brownell, Gladysheva et al.: "A Nedd8 conjugation pathway is essential for proteolytic targeting of p27Kip1 by ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 9, pp. 4579-84, 2000 (PubMed).

Hosts (24), (9)
Reactivities (30), (20), (19), (17), (16), (13), (12), (1)
Applications (21), (13), (10), (6), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (4), (1), (1)
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