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Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F) (C-Term) antibody

Details for Product No. ABIN184898, Supplier: Login to see New
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Synonyms NCE2, 2510010F15Rik, AI851109, RGD1307608, zgc:77005
(26), (14), (2), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
(52), (26), (18), (9), (9), (3), (1), (1)
(41), (21)
(3), (3), (3), (3), (3), (3), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(45), (41), (7), (6), (2), (2)
Pubmed 1 reference available
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Quantity 100 μg
Shipping to United States ( )
Availability Available
Immunogen Peptide with sequence C-RNKVDDYIKRYAR, from the C Terminus of the protein sequence according to NP_542409.1.
Blocking Peptide Blocking peptide for this product available: ABIN369801
Specificity This antibody is expected to recognise isoform 1 (NP_542409.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name UBE2F/NEDD8-conjugating enzyme (UBE2F Antibody Abstract)
Background Synonyms: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme
Gene ID 140739
NCBI Accession NP_542409
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Podust, Brownell, Gladysheva et al.: "A Nedd8 conjugation pathway is essential for proteolytic targeting of p27Kip1 by ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 9, pp. 4579-84, 2000 (PubMed).

Catalog No. ABIN184898
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