Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody

Details for Product No. ABIN184913
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Antigen
Synonyms TRIM28, FLA3, KAP-1, KAP-3, KAP3, SMAP, Smg-GDS, dJ190I16.1, KAP1, RNF96, TF1B, TIF1B, TIF1BETA, kap1, rnf96, tf1b, tif1b, AA408787, KRIP-1, Tif1b, Tif1beta, Kap1, Krip1
Epitope
C-Term
(16), (16), (13), (12), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(132), (58), (47), (15), (14), (1), (1), (1), (1), (1)
Host
Goat
(93), (30), (12)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(112), (58), (33), (32), (28), (23), (20), (18), (4), (3), (3), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN184913
374.27 $
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Immunogen Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KAP1 / TRIM28
Background Synonyms: TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein
Gene ID 10155
NCBI Accession NP_005753
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 120 kDa band observed in lysates of cell line HepG2 (calculated MW of 88.5 kDa according to NP_005753.1). The higher observed MW is explained by sumoylated protein. Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 4-6 µg/mL. Data obtained from a different batch (different goat). This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2010 Mar,17(3):821-8. (PMID: 19898899). Recommended concentration: 5 µg/mL. Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5 µg/mL (personal communication). Chromatin immunoprecipitation: This antibody has been successfully used in ChIP for K562 cells as described in the following paper: Frietze et al, PLoS One. 2010 Dec 8,5(12):e15082., PMID: 21170338.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody ABIN184913 (2 µg/mL) staining of HepG2 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (2) ABIN184913 (4 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Product cited in: Yokoe, Toiyama, Okugawa et al.: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, 2010 (PubMed).

General Friedman, Fredericks, Jensen et al.: "KAP-1, a novel corepressor for the highly conserved KRAB repression domain." in: Genes & development, Vol. 10, Issue 16, pp. 2067-78, 1996 (PubMed).

Validation Images
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