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Details for Product No. ABIN184913

Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody

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Synonyms kap1, rnf96, tf1b, tif1b, TIF1BETA, KAP1, RNF96, TF1B, TIF1B, AA408787, KAP-1, KRIP-1, Tif1b, Tif1beta, Kap1, Krip1, TRIM28
»Alternatives C-Term
»Alternatives Human, Mouse (Murine)
Clonality Polyclonal
»Alternatives Un-conjugated
Methode Type Peptide ELISA
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 2 references available
Catalog no. ABIN184913
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KAP1 / TRIM28
Background Synonyms: TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein
Gene ID 10155
NCBI Accession NP_005753.1
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 120 kDa band observed in lysates of cell line HepG2 (calculated MW of 88.5 kDa according to NP_005753.1). The higher observed MW is explained by sumoylated protein. Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 4-6 µg/mL. Data obtained from a different batch (different goat). This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2010 Mar,17(3):821-8. (PMID: 19898899). Recommended concentration: 5 µg/mL. Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5 µg/mL (personal communication). Chromatin immunoprecipitation: This antibody has been successfully used in ChIP for K562 cells as described in the following paper: Frietze et al, PLoS One. 2010 Dec 8,5(12):e15082., PMID: 21170338.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Yokoe, Toiyama, Okugawa et al.: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, 2010 (PubMed).

Friedman, Fredericks, Jensen et al.: "KAP-1, a novel corepressor for the highly conserved KRAB repression domain." in: Genes & development, Vol. 10, Issue 16, pp. 2067-78, 1996 (PubMed).

Alternatives for antigen "Tripartite Motif Containing 28 (TRIM28)", type "Antibodies"
Hosts (73), (26), (10)
Reactivities (109), (57), (46), (15), (15), (1), (1), (1), (1), (1)
Applications (89), (45), (24), (24), (20), (15), (14), (9), (3), (2), (2), (2), (1), (1)
Conjugates (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (16), (13), (7), (5), (2), (2), (2), (1), (1)