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Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody

Details for Product No. ABIN184913, Supplier: Login to see
Antigen
  • TRIM28
  • FLA3
  • KAP-1
  • KAP-3
  • KAP3
  • SMAP
  • Smg-GDS
  • dJ190I16.1
  • KAP1
  • RNF96
  • TF1B
  • TIF1B
  • TIF1BETA
  • kap1
  • rnf96
  • tf1b
  • tif1b
  • AA408787
  • KRIP-1
  • Tif1b
  • Tif1beta
  • Kap1
  • Krip1
Alternatives
anti-Human Tripartite Motif Containing 28 antibody for Western Blotting
Epitope
C-Term
30
22
18
15
14
13
11
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
206
70
52
5
3
1
1
1
1
1
Host
Goat
128
51
31
Clonality
Polyclonal
Conjugate
Un-conjugated
11
11
11
4
4
4
3
2
2
2
2
2
2
2
2
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
171
94
71
64
39
25
24
22
8
5
3
3
3
2
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.
Blocking Peptide Blocking peptide for this product available: ABIN369934
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KAP1 / TRIM28 (TRIM28 Antibody Abstract)
Background Synonyms: TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein
Gene ID 10155
NCBI Accession NP_005753
Pathways Hedgehog Signaling
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 120 kDa band observed in lysates of cell line HepG2 (calculated MW of 88.5 kDa according to NP_005753.1). The higher observed MW is explained by sumoylated protein. Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 4-6 µg/mL. Data obtained from a different batch (different goat). This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2010 Mar,17(3):821-8. (PMID: 19898899). Recommended concentration: 5 µg/mL. Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5 µg/mL (personal communication). Chromatin immunoprecipitation: This antibody has been successfully used in ChIP for K562 cells as described in the following paper: Frietze et al, PLoS One. 2010 Dec 8,5(12):e15082., PMID: 21170338.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN184913) ABIN184913 (2 µg/mL) staining of HepG2 lysate (35 µg protein in RIPA buffer). Primary...
 image for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN184913) ABIN184913 (4 µg/mL) staining of paraffin embedded Human Breast. Steamed antigen retr...
Product cited in: Yokoe, Toiyama, Okugawa et al.: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, 2010 (PubMed).

Background publications Friedman, Fredericks, Jensen et al.: "KAP-1, a novel corepressor for the highly conserved KRAB repression domain." in: Genes & development, Vol. 10, Issue 16, pp. 2067-78, 1996 (PubMed).