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ARIH2 antibody (protein ariadne-2 homolog) (C-Term)

Details for Product anti-ARIH2 Antibody No. ABIN184916, Supplier: Login to see New
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Synonyms ARI2, TRIAD1, AI843547, UIP48
(29), (15), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Human, Mouse (Murine)
(73), (20), (16), (5), (4), (3), (2), (2), (1), (1), (1), (1)
(47), (13), (13)
This ARIH2 antibody is un-conjugated
(3), (3), (3), (3), (3), (3), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(65), (40), (22), (13), (9), (5), (4), (3), (2)
Pubmed 2 references available
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Quantity 100 μg
Shipping to United States ( )
Availability Available
Immunogen Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.
Blocking Peptide Blocking peptide for this product available: ABIN369055
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ARIH2 / TRIAD1
Background Synonyms: ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2
Gene ID 10425, 23807
NCBI Accession NP_006312
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Customer's experiments gave a band at approx 60 kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8 kDa according to NP_006312.1). Recommended concentration: 1-3 µg/mL. Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. Immunoprecipitation: This antibody works well in IP.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Raz, Buijze, Raz et al.: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." in: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, 2014 (PubMed).

Background publications van der Reijden, Erpelinck-Verschueren, Löwenberg et al.: "TRIADs: a new class of proteins with a novel cysteine-rich signature." in: Protein science : a publication of the Protein Society, Vol. 8, Issue 7, pp. 1557-61, 1999 (PubMed).

Catalog No. ABIN184916
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