protein ariadne-2 homolog (ARIH2) (C-Term) antibody

Details for Product No. ABIN184916
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Antigen
Synonyms ARI2, TRIAD1, UIP48, AI843547
Epitope
C-Term
(6), (2), (2), (2), (1), (1)
Reactivity
Human, Mouse (Murine)
(36), (19), (18), (14), (14), (13), (3), (2)
Host
Goat
(28), (6), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(22), (12), (10), (5), (5), (2), (2), (2), (2)
Pubmed 1 reference available
Catalog no. ABIN184916
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ARIH2 / TRIAD1
Background Synonyms: ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2
Gene ID 10425, 23807
NCBI Accession NP_006312.1
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Customer's experiments gave a band at approx 60 kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8 kDa according to NP_006312.1). Recommended concentration: 1-3 µg/mL. Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. Immunoprecipitation: This antibody works well in IP.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General van der Reijden, Erpelinck-Verschueren, Löwenberg et al.: "TRIADs: a new class of proteins with a novel cysteine-rich signature." in: Protein science : a publication of the Protein Society, Vol. 8, Issue 7, pp. 1557-61, 1999 (PubMed).

Hosts (28), (6), (3)
Reactivities (36), (19), (18), (14), (14), (13), (3), (2)
Applications (22), (12), (10), (5), (5), (2), (2), (2), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (2), (2), (2), (1), (1)
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