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ARIH2 antibody (protein ariadne-2 homolog) (C-Term)

Details for Product anti-ARIH2 Antibody No. ABIN184916, Supplier: Login to see
Antigen
  • ARI2
  • TRIAD1
  • AI843547
  • UIP48
Epitope
C-Term
29
15
6
4
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
73
20
16
5
4
3
2
2
1
1
1
1
Host
Goat
47
13
13
Clonality
Polyclonal
Conjugate
This ARIH2 antibody is un-conjugated
3
3
3
3
3
3
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
65
40
22
13
9
5
4
3
2
Supplier
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Immunogen Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.
Blocking Peptide Blocking peptide for this product available: ABIN369055
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ARIH2 / TRIAD1
Background Synonyms: ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2
Gene ID 10425, 23807
NCBI Accession NP_006312
Pathways
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Customer's experiments gave a band at approx 60 kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8 kDa according to NP_006312.1). Recommended concentration: 1-3 µg/mL. Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. Immunoprecipitation: This antibody works well in IP.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Raz, Buijze, Raz et al.: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." in: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, 2014 (PubMed).

Background publications van der Reijden, Erpelinck-Verschueren, Löwenberg et al.: "TRIADs: a new class of proteins with a novel cysteine-rich signature." in: Protein science : a publication of the Protein Society, Vol. 8, Issue 7, pp. 1557-61, 1999 (PubMed).