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Sorting Nexin 16 (SNX16) (N-Term) antibody

Details for Product No. ABIN184967
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Antigen
Synonyms Snx16, zgc:77396, SNX16, snx16, LOC100230665, 4930522N22Rik, AI117577, AI747675
Epitope
N-Term
(4), (2), (1)
Reactivity
Human
(8), (5), (4), (4), (4), (3)
Host
Goat
(6), (2), (1)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(7), (6), (3), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184967
374.27 $
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Immunogen Peptide with sequence ATPYVPVPMPIGN-C, from the N Terminus of the protein sequence according to NP_071416.2, NP_690049.1, NP_690050.1.
Specificity This antibody is expected to recognise both isoform a (NP_071416.2, NP_690049.1) and isoform b (NP_690050.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SNX16
Background Synonyms: SNX16, sorting nexin 16, DKFZp666H147
Gene ID 64089, 74718, 64088
NCBI Accession NP_071416.2, NP_690049.1, NP_690050.1
Research Area Transporters
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38 kDa band observed in lysates of cell line Daudi (calculated MW of 39.2 kDa according to NP_071416.2). In transfected HEK293 transiently expressing SNX16 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.05-0.2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Sorting Nexin 16 (SNX16) (N-Term) antibody ABIN184967 (0.05µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Teasdale, Loci, Houghton et al.: "A large family of endosome-localized proteins related to sorting nexin 1." in: The Biochemical journal, Vol. 358, Issue Pt 1, pp. 7-16, 2001 (PubMed).

Validation Images
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