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Tripartite Motif Containing 2 (TRIM2) (C-Term) antibody

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Synonyms RNF86, mKIAA0517, narf
(34), (16), (15), (9), (8), (7), (2), (1)
Human, Mouse (Murine), Rat (Rattus)
(69), (20), (12), (5), (5), (1), (1), (1), (1)
(30), (24), (23)
(4), (4), (4), (4), (4), (4), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(71), (43), (18), (7), (6), (5), (2), (2), (2)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2, NP_001123539.1.
Blocking Peptide Blocking peptide for this product available: ABIN370095
Specificity This antibody is expected to recognise both reported isoforms (NP_056086.2, NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.
Cross-Reactivity Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TRIM2 (TRIM2 Antibody Abstract)
Background Synonyms: TRIM2, tripartite motif-containing 2, KIAA0517, tripartite motif protein 2, tripartite motif protein TRIM2, RNF86, tripartite motif protein TRIM2
Gene ID 23321, 80890
NCBI Accession NP_001123539, NP_056086
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 80+85 kDa bands observed in Rat Brain lysates (calculated MW of 81.5 kDa according to Human NP_001123539.1, 84.4 according to Human NP_056086.2 and 83.3 according to Rat NP_001102022.1). Recommended concentration: 0.5-2 µg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies. Recommended concentration: 6-12 µg/mL. Immunoprecipitation: This antibody was reported to work on Mouse protein in a publication (PMID: 21478148).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Reymond, Meroni, Fantozzi et al.: "The tripartite motif family identifies cell compartments." in: The EMBO journal, Vol. 20, Issue 9, pp. 2140-51, 2001 (PubMed).

Catalog No. ABIN184974
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