Tripartite Motif Containing 5 (TRIM5) (N-Term) antibody

Details for Product No. ABIN184983
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Antigen
Synonyms RNF88, TRIM5alpha, RGD1304579, Gm8833, EG667823, TRIM5
Epitope
N-Term
(25), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(81), (5), (2)
Host
Goat
(66), (12), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(69), (43), (30), (22), (10), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN184983
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1, NP_149083.1, NP_149084.1.
Specificity This antibody is expected to recognise all three human isoforms of this protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TRIM5 / RNF88
Background Synonyms: TRIM5, RNF88, tripartite motif-containing 5, TRIM5alpha, tripartite motif protein 5, tripartite motif protein TRIM5, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
Gene ID 85363
NCBI Accession NP_149023.1, NP_149083.1, NP_149084.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 38 kDa band observed in Human Plancenta and Human Uterus lysates (calculated MW of 37.7 kDa according to NP_149084.1). Recommended concentration: 2-6 µg/mL.
IHC: A previous batch was successfully used on Human Tonsil.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Tripartite Motif Containing 5 (TRIM5) (N-Term) antibody ABIN184983 staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Tripartite Motif Containing 5 (TRIM5) (N-Term) antibody (2) ABIN184983 (1 ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) positive cells next to a secondary follicle, B) positive cells below tonsillar epithelium.
General Reymond, Meroni, Fantozzi et al.: "The tripartite motif family identifies cell compartments." in: The EMBO journal, Vol. 20, Issue 9, pp. 2140-51, 2001 (PubMed).

Hosts (66), (12), (8)
Reactivities (81), (5), (2)
Applications (69), (43), (30), (22), (10), (4), (3), (2), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (25), (5), (4), (3), (2), (2), (1), (1), (1), (1)
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