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Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2) (C-Term) antibody

Details for Product No. ABIN185029, Supplier: Login to see
Antigen
  • FOXC2
  • Fkh14
  • Hfhbf3
  • MFH-1
  • Mfh1
  • Fkhl14
  • FKHL14
  • LD
  • MFH1
Epitope
C-Term
25
19
16
14
14
9
9
8
7
6
5
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
102
15
9
8
5
1
1
1
Host
Goat
57
28
19
1
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
5
5
5
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
96
75
32
25
7
5
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence RHAAPYSYDCTKY, from the C Terminus of the protein sequence according to NP_005242.1.
Blocking Peptide Blocking peptide for this product available: ABIN370423
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXC2 (FOXC2 Antibody Abstract)
Background Synonyms: FOXC2, forkhead box C2 (MFH-1, mesenchyme forkhead 1), MFH1, MFH-1, FKHL14, MFH-1,mesenchyme forkhead 1, forkhead (Drosophila)-like 14, forkhead, Drosophila, homolog-like 14, forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1), LD, forkhead box C2, forkhead, Drosophila, homolog-like 14
Gene ID 2303, 14234
NCBI Accession NP_005242
Research Area Organogenesis, Transcription Factors
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing full-length Human FOXC2 (myc and DYKDDDDK tagged), a band of approx. 65 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.1-0.2 µg/mL
IHC: In paraffin embedded Human Bresast cancer shows nuclear staning wirth some weak cytoplasm staining. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2) (C-Term) antibody (ABIN185029) ABIN185029 staining (1µg/ml) of Human Breast Cancer lysate (RIPA buffer, 30µg total p...
Western Blotting (WB) image for anti-Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2) (C-Term) antibody (ABIN185029) HEK293 lysate (10 µg protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDD...
Immunohistochemistry (IHC) image for anti-Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2) (C-Term) antibody (ABIN185029) ABIN185029 (2 µg/mL) staining of paraffin embedded Human Breast cancer. Steamed antig...
Background publications Brice, Mansour, Bell et al.: "Analysis of the phenotypic abnormalities in lymphoedema-distichiasis syndrome in 74 patients with FOXC2 mutations or linkage to 16q24." in: Journal of medical genetics, Vol. 39, Issue 7, pp. 478-83, 2002 (PubMed).