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Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2) (C-Term) antibody

Details for Product No. ABIN185029
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Antigen
Synonyms LD, MFH1, MFH-1, FKHL14, Fkh14, Fkhl14, Hfhbf3, FOXC2
Epitope
C-Term
(8), (7), (7), (7), (6), (6), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(74), (21), (18), (15), (13), (4)
Host
Goat
(40), (29), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(64), (46), (19), (15), (10), (7), (7), (4), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185029
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence RHAAPYSYDCTKY, from the C Terminus of the protein sequence according to NP_005242.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXC2
Background Synonyms: FOXC2, forkhead box C2 (MFH-1, mesenchyme forkhead 1), MFH1, MFH-1, FKHL14, MFH-1,mesenchyme forkhead 1, forkhead (Drosophila)-like 14, forkhead, Drosophila, homolog-like 14, forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1), LD, forkhead box C2, forkhead, Drosophila, homolog-like 14
Gene ID 2303, 14234
NCBI Accession NP_005242.1
Research Area Organogenesis, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing full-length Human FOXC2 (myc and DYKDDDDK tagged), a band of approx. 65 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.1-0.2 µg/mL
IHC: In paraffin embedded Human Bresast cancer shows nuclear staning wirth some weak cytoplasm staining. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2) (C-Term) antibody ABIN185029 staining (1µg/ml) of Human Breast Cancer lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2) (C-Term) antibody (2) ABIN185029 (2 µg/mL) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
anti-Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2) (C-Term) antibody (3) HEK293 lysate (10 µg protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed with ABIN185029 (0.1 µg/mL) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with ABIN185029 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
General Brice, Mansour, Bell et al.: "Analysis of the phenotypic abnormalities in lymphoedema-distichiasis syndrome in 74 patients with FOXC2 mutations or linkage to 16q24." in: Journal of medical genetics, Vol. 39, Issue 7, pp. 478-83, 2002 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185029
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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