Forkhead Box I1 (FOXI1) (C-Term) antibody

Details for Product No. ABIN185038
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Antigen
Synonyms FoxI1e, Xema, Xfoxi1a, ema, fkhl10, foxi1a, freac6, hfh3, xfoxi1, FOXI1, FKH10, FKHL10, FREAC-6, FREAC6, HFH-3, HFH3, 5830401E05Rik, Fkh10, Hfh3, foxi1e, xema, cb724, foo
Epitope
C-Term
(8), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(54), (21), (15), (14), (13), (13), (12), (1), (1)
Host
Goat
(40), (11), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(35), (18), (12), (10), (10), (6), (4), (3), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185038
374.27 $
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Immunogen Peptide with sequence C-TSGVLYPREGTEV, from the C Terminus of the protein sequence according to NP_036320.2, NP_658982.1.
Specificity This antibody is expected to recognise both reported human isoforms (NP_036320.2, NP_658982.1).
Cross-Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXI1 / FKHL10
Background Synonyms: FOXI1, forkhead box I1, HFH3, FKHL10, FREAC6, HNF-3/fork-head homolog-3, forkhead-related activator 6, forkhead (Drosophila)-like 10, Forkhead, drosophila, homolog-like 10, hepatocyte nuclear factor 3 forkhead homolog 3, FKH10, MGC34197, forkhead-like 10
Gene ID 2299
NCBI Accession NP_658982, NP_036320
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41.0 kDa according to NP_036320 (isoform a) and 30.8 kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide.
IHC: This product has been successfully used in Mouse Epididymus.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Pierrou, Hellqvist, Samuelsson et al.: "Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending." in: The EMBO journal, Vol. 13, Issue 20, pp. 5002-12, 1994 (PubMed).

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