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FOXL2 antibody (Forkhead Box L2) (C-Term)

Details for Product anti-FOXL2 Antibody No. ABIN185040, Supplier: Log in to see
Antigen
  • FOXL2
  • zgc:136549
  • foxl2
  • FoxL2
  • foxL2
  • AU045128
  • BPES
  • PINTO
  • Pfrk
  • BPES1
  • PFRK
  • POF3
Epitope
C-Term
29
18
16
14
2
1
1
1
1
1
1
Reactivity
Human
88
29
29
6
3
3
2
1
1
Host
Goat
71
14
6
Clonality
Polyclonal
Conjugate
This FOXL2 antibody is un-conjugated
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
68
35
16
12
7
4
3
2
1
1
Supplier
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Immunogen Peptide with sequence C-DSKTGALHSRLDL, from the C Terminus of the protein sequence according to NP_075555.1.
Blocking Peptide Blocking peptide for this product available: ABIN368927
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXL2 / BPES (FOXL2 Antibody Abstract)
Background Synonyms: FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, forkhead transcription factor FOXL2, blepharophimosis, epicanthus inversus and ptosis, Blepharophimosis, epicanthus inversus, and ptosis 1, POF3
Gene ID 668, 26927
NCBI Accession NP_075555
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50 kDa band observed in Human Ovary lysates (calculated MW of 38.8 kDa according to NP_075555.1). Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Ovary shows nuclear staining in the zona granulosa. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-FOXL2 antibody (Forkhead Box L2) (C-Term) (ABIN185040) ABIN185040 staining (1.5µg/ml) of Human Ovary extracts (RIPA buffer, 30µg total prote...
Product cited in: Padua, Fox, Jiang et al.: "Simultaneous gene deletion of gata4 and gata6 leads to early disruption of follicular development and germ cell loss in the murine ovary." in: Biology of reproduction, Vol. 91, Issue 1, pp. 24, 2014 (PubMed).

Blount, Schmidt, Justice et al.: "FoxL2 and Smad3 coordinately regulate follistatin gene transcription." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 7631-45, 2009 (PubMed).

Background publications Crisponi, Deiana, Loi et al.: "The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome." in: Nature genetics, Vol. 27, Issue 2, pp. 159-66, 2001 (PubMed).