Forkhead Box L2 (FOXL2) (C-Term) antibody

Details for Product No. ABIN185040
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Antigen
Synonyms FOXL2, zgc:136549, foxl2, FoxL2, foxL2, AU045128, BPES, PINTO, Pfrk, BPES1, PFRK, POF3
Epitope
C-Term
(18), (17), (8), (4), (2), (1), (1), (1), (1)
Reactivity
Human
(70), (36), (35), (26), (25), (13), (1), (1)
Host
Goat
(64), (5), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(45), (20), (17), (10), (8), (4), (2), (1), (1)
Pubmed 2 references available
Quantity 100 µg
Options
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Catalog No. ABIN185040
374.27 $
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  • +1 404 474 4654
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Immunogen Peptide with sequence C-DSKTGALHSRLDL, from the C Terminus of the protein sequence according to NP_075555.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXL2 / BPES
Background Synonyms: FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, forkhead transcription factor FOXL2, blepharophimosis, epicanthus inversus and ptosis, Blepharophimosis, epicanthus inversus, and ptosis 1, POF3
Gene ID 668, 26927
NCBI Accession NP_075555
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50 kDa band observed in Human Ovary lysates (calculated MW of 38.8 kDa according to NP_075555.1). Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Human Ovary shows nuclear staining in the zona granulosa. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Forkhead Box L2 (FOXL2) (C-Term) antibody ABIN185040 staining (1.5µg/ml) of Human Ovary extracts (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product cited in: Blount, Schmidt, Justice et al.: "FoxL2 and Smad3 coordinately regulate follistatin gene transcription." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 7631-45, 2009 (PubMed).

General Crisponi, Deiana, Loi et al.: "The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome." in: Nature genetics, Vol. 27, Issue 2, pp. 159-66, 2001 (PubMed).

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