SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody

Details for Product No. ABIN185082
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Antigen
Synonyms AOS1, CDGAP, HSPC140, SUA1, UBLE1A, 2400010M20Rik, 2610044L12Rik, AL033372, AW743391, D7Ertd177e, Sua1, Uble1a, Aos1p, Sua1p, aos
Epitope
C-Term
(20), (6), (6), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(89), (28), (25), (7), (7), (3)
Host
Goat
(74), (10), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(79), (59), (26), (20), (11), (11), (3), (2), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185082
374.27 $
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Immunogen Peptide with sequence MKGNGIVECLGPK, from the C Terminus of the protein sequence according to NP_005491.1.
Cross-Reactivity Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SAE1 / AOS1
Background Synonyms: SAE1, AOS1, SUA1, HSPC140, SUMO-1 activating enzyme subunit 1, sentrin/SUMO-activating protein AOS1, SUMO-1 activating enzyme E1 N subunit, ubiquitin-like protein SUMO-1 activating enzyme, SUMO1 activating enzyme subunit 1, FLJ3091, UBLE1A, activator of SUMO1, ubiquitin-like 1-activating enzyme E1A
Gene ID 10055
NCBI Accession NP_005491
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 40 kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40 kDa according to NP_005491). Recommended for use at 1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term) antibody ABIN185082 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

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