ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) (C-Term) antibody

Details for Product No. ABIN185100
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Antigen
Synonyms CENTG3, AGAP-3, CRAG, MRIP-1, cnt-g3, AV028425, Centg3, Crag, Crag.
Epitope
C-Term
(9), (7), (5), (2), (1), (1), (1), (1)
Reactivity
Human
(26), (6), (6), (1), (1), (1), (1), (1)
Host
Goat
(17), (7), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(18), (14), (5), (5), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185100
374.27 $
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Immunogen Peptide with sequence C-NPSAELHRSPSLL, from the C Terminus of the protein sequence according to NP_114152.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CENTG3 / MRIP1
Background Synonyms: AGAP3, CENTG3, MRIP1, centaurin, gamma 3, MRIP-1, MRIP-1 protein, ArfGAP with GTPase domain, ankyrin repeat and PH domain 3, CRAG, FLJ16146, FLJ34452, CRMP (collapsin response mediator protein) associated, centaurin, gamma 3
Gene ID 116988, 213990
NCBI Accession NP_114152
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 45 kDa band in Human Brain and Human Liver lysates at 1 µg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152.
IHC: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) (C-Term) antibody ABIN185100 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Surks, Richards, Mendelsohn: "Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51484-93, 2003 (PubMed).

Validation Images
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