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Details for Product No. ABIN185100

ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) (C-Term) antibody

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Antigen
Synonyms AGAP-3, CENTG3, CRAG, MRIP-1, cnt-g3, AV028425, Centg3, Crag, Crag.
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN185100
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Availability Ships within 5 Business Days
Immunogen Peptide with sequence C-NPSAELHRSPSLL, from the C Terminus of the protein sequence according to NP_114152.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CENTG3 / MRIP1
Background Synonyms: AGAP3, CENTG3, MRIP1, centaurin, gamma 3, MRIP-1, MRIP-1 protein, ArfGAP with GTPase domain, ankyrin repeat and PH domain 3, CRAG, FLJ16146, FLJ34452, CRMP (collapsin response mediator protein) associated, centaurin, gamma 3
Gene ID 116988, 213990
NCBI Accession NP_114152.3
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 45 kDa band in Human Brain and Human Liver lysates at 1 µg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152.
IHC: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Surks, Richards, Mendelsohn: "Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51484-93, 2003 (PubMed).

Alternatives for antigen "ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3)", type "Antibodies"
Hosts (8), (8), (1)
Reactivities (16), (5), (5)
Applications (8), (8), (4), (2), (1), (1)
Epitopes (5), (3), (2), (1), (1)
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