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ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) (C-Term) antibody

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Synonyms CENTG3, AGAP-3, CRAG, MRIP-1, cnt-g3, AV028425, Centg3, Crag, Crag.
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(27), (19), (1)
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
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Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-NPSAELHRSPSLL, from the C Terminus of the protein sequence according to NP_114152.3.
Blocking Peptide Blocking peptide for this product available: ABIN369489
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CENTG3 / MRIP1 (AGAP3 Antibody Abstract)
Background Synonyms: AGAP3, CENTG3, MRIP1, centaurin, gamma 3, MRIP-1, MRIP-1 protein, ArfGAP with GTPase domain, ankyrin repeat and PH domain 3, CRAG, FLJ16146, FLJ34452, CRMP (collapsin response mediator protein) associated, centaurin, gamma 3
Gene ID 116988, 213990
NCBI Accession NP_114152
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 45 kDa band in Human Brain and Human Liver lysates at 1 µg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152.
IHC: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) (C-Term) antibody (ABIN185100) ABIN185100 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retri...
Background publications Surks, Richards, Mendelsohn: "Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51484-93, 2003 (PubMed).

Catalog No. ABIN185100
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