rho GTPase Activating Protein 26 (ARHGAP26) (C-Term) antibody

Details for Product No. ABIN185109
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Antigen
Synonyms ARHGAP26, GRAF, GRAF1, OPHN1L, OPHN1L1, 1810044B20Rik, 2610010G17Rik, 4933432P15Rik, AI853435, mKIAA0621
Epitope
C-Term
(10), (7), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(47), (16), (15), (13), (13), (12), (12)
Host
Goat
(40), (5), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(30), (23), (15), (10), (7), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185109
374.27 $
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Immunogen Peptide with sequence C-KTGLIPENYVEFL, from the C Terminus of the protein sequence according to NP_055886.1, NP_001129080.1.
Specificity This antibody is expected to recognise both reported isoforms.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GRAF / ARHGAP26
Background Synonyms: ARHGAP26, GRAF, Rho GTPase activating protein 26, KIAA0621, FLJ42530, OPHN1L, OPHN1L1, GTPase regulator associated with FAK, GTPase regulator associated with focal adhesion kinase pp125(FAK), GTPase regulator associated with the focal adhesion kinase pp125
Gene ID 23092, 71302
NCBI Accession NP_055886, NP_001129080
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 90 kDa band observed in Human Heart lysates (calculated MW of 92.2 kDa according to NP_055886.1). Recommended concentration: 0.3-1 µg/mL. An additional strong band of unknown identity was also consistently observed at 16 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-rho GTPase Activating Protein 26 (ARHGAP26) (C-Term) antibody ABIN185109 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
General Borkhardt, Bojesen, Haas et al.: "The human GRAF gene is fused to MLL in a unique t(5;11)(q31;q23) and both alleles are disrupted in three cases of myelodysplastic syndrome/acute myeloid leukemia with a deletion 5q." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 16, pp. 9168-73, 2000 (PubMed).

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