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GRAF antibody (rho GTPase Activating Protein 26) (C-Term)

Details for Product anti-ARHGAP26 Antibody No. ABIN185109, Supplier: Log in to see
Antigen
  • ARHGAP26
  • GRAF
  • GRAF1
  • OPHN1L
  • OPHN1L1
  • 1810044B20Rik
  • 2610010G17Rik
  • 4933432P15Rik
  • AI853435
  • mKIAA0621
Epitope
C-Term
28
11
9
2
2
2
1
1
1
1
1
1
Reactivity
Human
82
25
23
3
3
1
1
Host
Goat
64
14
4
Clonality
Polyclonal
Conjugate
This GRAF antibody is un-conjugated
6
6
6
5
5
5
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
52
48
31
11
6
2
1
Supplier
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Immunogen Peptide with sequence C-KTGLIPENYVEFL, from the C Terminus of the protein sequence according to NP_055886.1, NP_001129080.1.
Blocking Peptide Blocking peptide for this product available: ABIN369375
Specificity This antibody is expected to recognise both reported isoforms.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GRAF / ARHGAP26 (ARHGAP26 Antibody Abstract)
Background Synonyms: ARHGAP26, GRAF, Rho GTPase activating protein 26, KIAA0621, FLJ42530, OPHN1L, OPHN1L1, GTPase regulator associated with FAK, GTPase regulator associated with focal adhesion kinase pp125(FAK), GTPase regulator associated with the focal adhesion kinase pp125
Gene ID 23092, 71302
NCBI Accession NP_055886, NP_001129080
Pathways
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 90 kDa band observed in Human Heart lysates (calculated MW of 92.2 kDa according to NP_055886.1). Recommended concentration: 0.3-1 µg/mL. An additional strong band of unknown identity was also consistently observed at 16 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-GRAF antibody (rho GTPase Activating Protein 26) (C-Term) (ABIN185109) ABIN185109 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) wi...
Background publications Borkhardt, Bojesen, Haas et al.: "The human GRAF gene is fused to MLL in a unique t(5;11)(q31;q23) and both alleles are disrupted in three cases of myelodysplastic syndrome/acute myeloid leukemia with a deletion 5q." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 16, pp. 9168-73, 2000 (PubMed).