SHFM1 antibody (C-Term)

Catalog No. ABIN185118

Product Details anti-SHFM1 Antibody

Target: SHFM1 (Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SHFM1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC)

Purpose: DSS1 / SHFM1

Sequence: RAELEKHGYK METS

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RAELEKHGYKMETS, from the C Terminus of the protein sequence according to NP_006295.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1 μg/mL. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SHFM1

Target: SHFM1 (Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1))

Alternative Name: SHFM1 (SHFM1 Products)

Background: SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot

Gene ID: 7979, 20422

NCBI Accession: NP_006295