SHFM1 antibody (C-Term)

Catalog No. ABIN185118

Product Details anti-SHFM1 Antibody

Target: SHFM1 (Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SHFM1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC)

Purpose: DSS1 / SHFM1

Sequence: RAELEKHGYK METS

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RAELEKHGYKMETS, from the C Terminus of the protein sequence according to NP_006295.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1 μg/mL. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SHFM1

Target: SHFM1 (Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1))

Alternative Name: SHFM1 (SHFM1 Products)

Synonyms: DSS1 antibody, ECD antibody, SEM1 antibody, SHFD1 antibody, SHSF1 antibody, Shfdg1 antibody, Shfg antibody, Dss1 antibody, SHFM1 antibody, dss1 antibody, ecd antibody, sem1 antibody, shfd1 antibody, shfdg1 antibody, shfm1 antibody, shfm1.S antibody, shsf1 antibody, wu:fb55c02 antibody, zgc:56203 antibody, zgc:85901 antibody, SEM1, 26S proteasome complex subunit antibody, SEM1, 26S proteasome complex subunit S homeolog antibody, SEM1 antibody, Sem1 antibody, sem1.S antibody, sem1 antibody

Background: SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot

Gene ID: 7979, 20422

NCBI Accession: NP_006295