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Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1) (C-Term) antibody

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Synonyms Dss1, DSS1, ECD, SEM1, SHFD1, SHSF1, Shfdg1, Shfg
(13), (12), (7), (1)
Human, Mouse (Murine), Dog (Canine), Cow (Bovine), Pig (Porcine)
(39), (7), (4), (3), (2), (2), (1)
(26), (15)
(3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(35), (16), (6), (6), (4), (2), (2)
Pubmed 1 reference available
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Quantity 100 μg
Shipping to United States ( )
Availability Available
Immunogen Peptide with sequence C-RAELEKHGYKMETS, from the C Terminus of the protein sequence according to NP_006295.1.
Blocking Peptide Blocking peptide for this product available: ABIN369734
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DSS1 / SHFM1 (SHFM1 Antibody Abstract)
Background Synonyms: SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot 1, split hand/foot malformation type 1
Gene ID 7979, 20422
NCBI Accession NP_006295
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1 µg/mL. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx. 8 kDa according to NP_006295. Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Wei, Trempus, Cannon et al.: "Identification of Dss1 as a 12-O-tetradecanoylphorbol-13-acetate-responsive gene expressed in keratinocyte progenitor cells, with possible involvement in early skin tumorigenesis." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 1758-68, 2003 (PubMed).

Catalog No. ABIN185118
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