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Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1) (C-Term) antibody

Details for Product No. ABIN185118, Supplier: Login to see
Antigen
  • Dss1
  • DSS1
  • ECD
  • SEM1
  • SHFD1
  • SHSF1
  • Shfdg1
  • Shfg
Epitope
C-Term
13
12
7
1
Reactivity
Human, Mouse (Murine), Dog (Canine), Cow (Bovine), Pig (Porcine)
39
7
4
3
2
2
1
Host
Goat
26
15
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
35
16
6
6
4
2
2
Supplier
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Immunogen Peptide with sequence C-RAELEKHGYKMETS, from the C Terminus of the protein sequence according to NP_006295.1.
Blocking Peptide Blocking peptide for this product available: ABIN369734
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DSS1 / SHFM1 (SHFM1 Antibody Abstract)
Background Synonyms: SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot 1, split hand/foot malformation type 1
Gene ID 7979, 20422
NCBI Accession NP_006295
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1 µg/mL. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx. 8 kDa according to NP_006295. Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Wei, Trempus, Cannon et al.: "Identification of Dss1 as a 12-O-tetradecanoylphorbol-13-acetate-responsive gene expressed in keratinocyte progenitor cells, with possible involvement in early skin tumorigenesis." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 1758-68, 2003 (PubMed).