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Details for Product No. ABIN185118

Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1) (C-Term) antibody

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Antigen
Synonyms ECD, DSS1, SEM1, SHFD1, SHSF1, Shfdg1, Shfg, Dss1
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine), Dog (Canine), Cow (Bovine), Pig (Porcine)
Host
Clonality Polyclonal
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN185118
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Product details

Target Details Application Details Handling References Alternatives back to top
Immunogen Peptide with sequence C-RAELEKHGYKMETS, from the C Terminus of the protein sequence according to NP_006295.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Target Details

Application Details Handling References Alternatives back to top
Alternative Name DSS1 / SHFM1
Background Synonyms: SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot 1, split hand/foot malformation type 1
Gene ID 7979, 20422
NCBI Accession NP_006295.1

Application Details

Target Details Handling References Alternatives back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1 µg/mL. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx. 8 kDa according to NP_006295. Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.
Restrictions For Research Use only

Handling

Target Details Application Details References Alternatives back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References

Target Details Application Details Handling Alternatives back to top
Wei, Trempus, Cannon et al.: "Identification of Dss1 as a 12-O-tetradecanoylphorbol-13-acetate-responsive gene expressed in keratinocyte progenitor cells, with possible involvement in early skin tumorigenesis." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 1758-68, 2003 (PubMed).

Alternatives

Target Details Application Details Handling References back to top
Alternatives for antigen "Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1)", type "Antibodies"
Hosts (10), (6)
Reactivities (13), (2), (2), (1), (1)
Applications (14), (6), (4), (3), (1), (1), (1)
Epitopes (4), (2)
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