Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1) (C-Term) antibody

Details for Product No. ABIN185154
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Synonyms 4E-T, Clast4, AA410001, AU021239, D11Ertd166e, 2610509L04Rik, A930019J01Rik, RGD1560908, eif4enif1, MGC80355, EIF4ENIF1, wu:fd15a05, zgc:101646, MGC147579
(5), (5), (4), (2), (1)
(30), (3), (3), (3), (2), (2)
(18), (9), (7)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(31), (11), (4), (4), (4), (3), (3), (2), (2)
Pubmed 1 reference available
Catalog no. ABIN185154
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-AKVISVDELEYRQ, from the C Terminus of the protein sequence according to NP_062817.2, NP_001157974.1.
Specificity This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2, NP_001157973.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name 4E-T / EIF4ENIF1
Background Synonyms: EIF4ENIF1, 4E-T, Clast4, FLJ21601, 2610509L04Rik, eukaryotic translation initiation factor 4E nuclear import factor 1, eIF4E-transporter, FLJ26551, OTTHUMP00000063276
Gene ID 56478, 74203
NCBI Accession NP_062817.2, NP_001157974.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 140-150 kDa band observed in 293 lysates (predicted size of approx. 108 kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5 µg/mL
IHC: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1) (C-Term) antibody ABIN185154 staining (0.25µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1) (C-Term) antibody (2) ABIN185154 (5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Dostie, Ferraiuolo, Pause et al.: "A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E." in: The EMBO journal, Vol. 19, Issue 12, pp. 3142-56, 2000 (PubMed).

Hosts (18), (9), (7)
Reactivities (30), (3), (3), (3), (2), (2)
Applications (31), (11), (4), (4), (4), (3), (3), (2), (2)
Epitopes (5), (5), (4), (2), (1)
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