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Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) (C-Term) antibody

Details for Product No. ABIN185182, Supplier: Login to see
Antigen
  • VPS35
  • 23.m05999
  • DKFZp459E1510
  • CG5625
  • DVps35
  • Dmel\\CG5625
  • Dvps35
  • dvps35
  • vps35
  • MGC81959
  • bZ34G2.3
  • si:rp71-34g2.3
  • wu:fa66e03
  • wu:fb95c08
  • zgc:136268
  • AI647796
  • Mem3
  • MEM3
  • PARK17
Epitope
C-Term
19
3
3
2
2
1
1
Reactivity
Human
33
12
9
5
1
1
1
1
1
Host
Goat
19
12
3
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
33
19
18
14
8
3
2
1
1
1
Supplier
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Immunogen Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
Blocking Peptide Blocking peptide for this product available: ABIN369911
Specificity Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name VPS35 / MEM3 (vps35 Antibody Abstract)
Background Synonyms: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35
Gene ID 55737, 65114
NCBI Accession NP_060676
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 85 kDa band observed in Human Brain Cerebellum lysates (predicted MW of 91 kDa according to NP_060676.2). Recommended for use at 0.01-1 µg/mL.
IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis. Data obtained with a previous batch. Immunofluorescence: Staining of VPS35 (red) in human melanoma cells 1205LU. Please see Scott KL et al., Nature. 2009 Jun 25,459(7250):1085-90., PMID: 19553991. Data obtained with a previous batch.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) (C-Term) antibody (ABIN185182) ABIN185182 staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein...
Immunohistochemistry (IHC) image for anti-Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) (C-Term) antibody (ABIN185182) ABIN185182 staining of paraffin embedded Human Testis showing A) Epithelial cells of ...
Product cited in: Lee, Uchida, Emoto et al.: "Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 17, Issue 8, pp. 728-36, 2012 (PubMed).

Background publications Zhang, Yu, Gao et al.: "Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21." in: Genomics, Vol. 70, Issue 2, pp. 253-7, 2001 (PubMed).