Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) (C-Term) antibody

Details for Product No. ABIN185182
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Synonyms Mem3, MEM3, PARK17, VPS35, 23.m05999, DKFZp459E1510, CG5625, DVps35, Dmel\\CG5625, Dvps35, dvps35, vps35, MGC81959, bZ34G2.3, si:rp71-34g2.3, wu:fa66e03, wu:fb95c08, zgc:136268
(6), (3), (2), (2)
(16), (6), (5), (4), (1)
(8), (8), (2)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(17), (8), (6), (3), (3), (3)
Pubmed 2 references available
Catalog no. ABIN185182
Quantity 100 µg
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Immunogen Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
Specificity Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name VPS35 / MEM3
Background Synonyms: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35
Gene ID 55737, 65114
NCBI Accession NP_060676.2
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 85 kDa band observed in Human Brain Cerebellum lysates (predicted MW of 91 kDa according to NP_060676.2). Recommended for use at 0.01-1 µg/mL.
IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis. Data obtained with a previous batch. Immunofluorescence: Staining of VPS35 (red) in human melanoma cells 1205LU. Please see Scott KL et al., Nature. 2009 Jun 25,459(7250):1085-90., PMID: 19553991. Data obtained with a previous batch.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) (C-Term) antibody ABIN185182 staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) (C-Term) antibody (2) ABIN185182 staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3 ug/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining.
Product cited in: Lee, Uchida, Emoto et al.: "Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 17, Issue 8, pp. 728-36, 2012 (PubMed).

General Zhang, Yu, Gao et al.: "Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21." in: Genomics, Vol. 70, Issue 2, pp. 253-7, 2001 (PubMed).

Hosts (8), (8), (2)
Reactivities (16), (6), (5), (4), (1)
Applications (17), (8), (6), (3), (3), (3)
Epitopes (6), (3), (2), (2)
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