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Peroxisome Proliferator-Activated Receptor delta (PPARD) (C-Term) antibody

Details for Product No. ABIN185282, Supplier: Login to see
Antigen
  • PPARD
  • NUC-1
  • NUC1
  • Nr1c2
  • PPAR-beta
  • PPAR-delta
  • PPAR[b]
  • PPARdelta
  • Pparb
  • Pparb/d
  • FAAR
  • NR1C2
  • NUCI
  • NUCII
  • PPARB
  • PPARBETA
  • ppard
  • pparb
Alternatives
anti-Rat (Rattus) Peroxisome Proliferator-Activated Receptor delta antibody for Enzyme Immunoassay
Epitope
C-Term
57
16
15
13
10
9
5
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
129
50
28
9
8
7
7
5
3
2
1
1
1
1
1
1
1
Host
Goat
121
22
12
Clonality
Polyclonal
Conjugate
Un-conjugated
10
8
5
5
5
5
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
134
94
20
19
17
10
8
6
6
3
2
2
1
1
1
Supplier
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Immunogen Peptide with sequence CHPLLQEIYKDMY, from the C Terminus of the protein sequence according to NP_006229.1.
Blocking Peptide Blocking peptide for this product available: ABIN369191
Specificity This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
Cross-Reactivity Mouse (Murine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PPAR delta (PPARD Antibody Abstract)
Background Synonyms: PPARD, FAAR, NUC1, NUCI, NR1C2, NUCII, PPARB, MGC3931, PPAR-beta, peroxisome proliferative activated receptor, delta, nuclear hormone receptor 1, peroxisome proliferator-activated receptor delta, OTTHUMP00000016256, OTTHUMP00000016257
Molecular Weight 49.9kDa
Gene ID 5467
NCBI Accession NP_006229
Pathways Nuclear Receptor Transcription Pathway
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55 kDa band observed in Human Lymphoblastoid and T cell line MOLT4 lysates (calculated MW of 49.9 kDa according to NP_006229.1). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Peroxisome Proliferator-Activated Receptor delta (PPARD) (C-Term) antibody (ABIN185282) ABIN185282 (0.3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primar...
Product cited in: Minamiyama, Takemura, Kodai et al.: "Iron restriction improves type 2 diabetes mellitus in Otsuka Long-Evans Tokushima fatty rats." in: American journal of physiology. Endocrinology and metabolism, Vol. 298, Issue 6, pp. E1140-9, 2010 (PubMed).

Background publications Park, Vogelstein, Kinzler: "Genetic disruption of PPARdelta decreases the tumorigenicity of human colon cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 5, pp. 2598-603, 2001 (PubMed).