Peroxisome Proliferator-Activated Receptor delta (PPARD) (C-Term) antibody

Details for Product No. ABIN185282
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Antigen
Synonyms PPARD, NUC-1, NUC1, Nr1c2, PPAR-beta, PPAR-delta, PPAR[b], PPARdelta, Pparb, Pparb/d, FAAR, NR1C2, NUCI, NUCII, PPARB, PPARBETA, ppard, pparb
Epitope
C-Term
(24), (9), (8), (7), (7), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(67), (30), (15), (8), (7), (6), (5), (4), (3), (2), (1), (1)
Host
Goat
(64), (13), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(82), (56), (19), (10), (9), (8), (7), (2), (1)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN185282
374.27 $
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Immunogen Peptide with sequence CHPLLQEIYKDMY, from the C Terminus of the protein sequence according to NP_006229.1.
Specificity This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
Cross-Reactivity Mouse (Murine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PPAR delta
Background Synonyms: PPARD, FAAR, NUC1, NUCI, NR1C2, NUCII, PPARB, MGC3931, PPAR-beta, peroxisome proliferative activated receptor, delta, nuclear hormone receptor 1, peroxisome proliferator-activated receptor delta, OTTHUMP00000016256, OTTHUMP00000016257
Molecular Weight 49.9kDa
Gene ID 5467
NCBI Accession NP_006229
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55 kDa band observed in Human Lymphoblastoid and T cell line MOLT4 lysates (calculated MW of 49.9 kDa according to NP_006229.1). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Peroxisome Proliferator-Activated Receptor delta (PPARD) (C-Term) antibody ABIN185282 (0.3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product cited in: Minamiyama, Takemura, Kodai et al.: "Iron restriction improves type 2 diabetes mellitus in Otsuka Long-Evans Tokushima fatty rats." in: American journal of physiology. Endocrinology and metabolism, Vol. 298, Issue 6, pp. E1140-9, 2010 (PubMed).

General Park, Vogelstein, Kinzler: "Genetic disruption of PPARdelta decreases the tumorigenicity of human colon cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 5, pp. 2598-603, 2001 (PubMed).

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