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ADAM12 antibody (ADAM Metallopeptidase Domain 12) (N-Term)

Details for Product anti-ADAM12 Antibody No. ABIN185301, Supplier: Login to see
Antigen
  • ADAM12
  • adam12
  • Mltna
  • mKIAA4001
  • MCMP
  • MCMPMltna
  • MLTN
  • MLTNA
Alternatives
anti-Human ADAM12 antibody for Western Blotting
Epitope
N-Term
26
14
6
4
3
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
75
23
21
2
1
1
Host
Goat
43
31
9
5
Clonality
Polyclonal
Conjugate
This ADAM12 antibody is un-conjugated
5
3
3
3
3
3
2
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
67
44
26
9
9
6
4
2
1
1
Supplier
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Immunogen Peptide with sequence AARPLPVSPARALC, from the N Terminus of the protein sequence according to NP_003465.3, NP_067673.2.
Blocking Peptide Blocking peptide for this product available: ABIN370279
Specificity This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ADAM12 (ADAM12 Antibody Abstract)
Background Synonyms: ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha, A disintegrin and metalloproteinase domain 12 (Meltrin-alpha, mouse, homolog of), ADAM metallopeptidase domain 12, RP11-295J3.5, ADAM metallopeptidase domain 12
Gene ID 8038
NCBI Accession NP_003465, NP_067673
Research Area Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Proteases
Pathways EGFR Signaling Pathway
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 90 kDa band observed in human heart lysates (calculated MW of 99.6 kDa according to NP_003465 and 80.5 kDa according to NP_067673). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Prostate shows strong cytoplasm staining of a group of secretory epithelial cells. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Tanaka, Nanba, Mori et al.: "ADAM binding protein Eve-1 is required for ectodomain shedding of epidermal growth factor receptor ligands." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 41950-9, 2004 (PubMed).

Galliano, Huet, Frygelius et al.: "Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion." in: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13933-9, 2000 (PubMed).

Gilpin, Loechel, Mattei et al.: "A novel, secreted form of human ADAM 12 (meltrin alpha) provokes myogenesis in vivo." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 157-66, 1998 (PubMed).