ADAM Metallopeptidase Domain 12 (ADAM12) (N-Term) antibody

Details for Product No. ABIN185301
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Antigen
Synonyms ADAM12, adam12, Mltna, mKIAA4001, MCMP, MCMPMltna, MLTN, MLTNA
Epitope
N-Term
(9), (8), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(67), (34), (29), (26), (26), (26), (25)
Host
Goat
(48), (11), (8), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(40), (23), (20), (12), (12), (8), (6), (2), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 100 μg
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Catalog No. ABIN185301
374.27 $
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Immunogen Peptide with sequence AARPLPVSPARALC, from the N Terminus of the protein sequence according to NP_003465.3, NP_067673.2.
Specificity This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ADAM12
Background Synonyms: ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha, A disintegrin and metalloproteinase domain 12 (Meltrin-alpha, mouse, homolog of), ADAM metallopeptidase domain 12, RP11-295J3.5, ADAM metallopeptidase domain 12
Gene ID 8038
NCBI Accession NP_003465, NP_067673
Research Area Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Proteases
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 90 kDa band observed in human heart lysates (calculated MW of 99.6 kDa according to NP_003465 and 80.5 kDa according to NP_067673). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Prostate shows strong cytoplasm staining of a group of secretory epithelial cells. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Gilpin, Loechel, Mattei et al.: "A novel, secreted form of human ADAM 12 (meltrin alpha) provokes myogenesis in vivo." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 157-66, 1998 (PubMed).

Galliano, Huet, Frygelius et al.: "Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion." in: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13933-9, 2000 (PubMed).

Tanaka, Nanba, Mori et al.: "ADAM binding protein Eve-1 is required for ectodomain shedding of epidermal growth factor receptor ligands." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 41950-9, 2004 (PubMed).

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