Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) (Internal Region) antibody

Details for Product No. ABIN185307
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Antigen
Synonyms lrp6-A, Xlrp6, adcad2, lrp6, LRP6, ADCAD2, C030016K15Rik, Cd, Gw
Epitope
Internal Region
(13), (13), (12), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(74), (43), (42), (41), (41), (31), (12), (12), (1)
Host
Goat
(71), (4), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(36), (34), (21), (19), (13), (10), (3), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN185307
374.27 $
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Immunogen Peptide with sequence CMTSVATAKGYTSDL, from the internal region of the protein sequence according to NP_002327.2.
Specificity This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LRP6
Background Synonyms: LRP6, low density lipoprotein receptor-related protein 6, low density lipoprotein-related protein 6
Gene ID 4040, 16974
NCBI Accession NP_002327
Research Area Stem Cells, Wnt
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 50 kDa and 35 kDa in Human Adipose lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180 kDa according to NP_002327.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration: 4-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) (Internal Region) antibody ABIN185307 (5µg/ml) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Tamai, Semenov, Kato et al.: "LDL-receptor-related proteins in Wnt signal transduction." in: Nature, Vol. 407, Issue 6803, pp. 530-5, 2000 (PubMed).

Giles, van Es, Clevers: "Caught up in a Wnt storm: Wnt signaling in cancer." in: Biochimica et biophysica acta, Vol. 1653, Issue 1, pp. 1-24, 2003 (PubMed).

Validation Images
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