ADH1A/1B/1C (N-Term) antibody
Verified
Reactivity: Human
IHC, ELISA, WB
Host: Goat
Polyclonal
unconjugated
2 images
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- Target
- ADH1A/1B/1C
- Binding Specificity
- N-Term
- Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
- Purpose
- pan ADH
- Sequence
- STAGKVMKCK A
- Specificity
- This antibody is expected to recognise the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of human alcohol dehydrogenase.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1, NP_000659.2, NP_000660.1.
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: Customer found this product to work in IHC on Human Liver.
Western Blot: Approx 38 kDa band observed in Human Liver lysates (calculated MW of 39.9 kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 1-3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:8000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- ADH1A/1B/1C
- Alternative Name
- ADH1A, B, C
- Background
- Pan ADH, ADH1B, ADH2, alcohol dehydrogenase IB (class I), beta polypeptide, ADH, beta subunit, aldehyde reductase, alcohol dehydrogenase 2, alcohol, ADH, gamma subunit, alcohol dehydrogenase 3 (class I), gamma polypeptide dehydrogenase 2 (class I), beta p
- Gene ID
- 124, 125, 126
- NCBI Accession
- NP_000658, NP_000659, NP_000660
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