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Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E) (C-Term) antibody

Details for Product No. ABIN185359, Supplier: Log in to see
Antigen
  • M-SEMAH
  • M-SemaK
  • SEMAH
  • coll-5
  • 6430702L12
  • AA408817
  • Semah
  • mKIAA0331
Epitope
C-Term
25
14
5
5
5
1
1
1
1
Reactivity
Human
70
27
18
5
4
3
2
2
2
1
Host
Goat
42
35
4
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
5
4
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
45
33
31
12
11
5
3
3
2
2
Supplier
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Immunogen Peptide with sequence C-KPEHYRLPRHTLDS, from the C Terminus of the protein sequence according to NP_036563.1.
Blocking Peptide Blocking peptide for this product available: ABIN369328
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SEMA3E (SEMA3E Antibody Abstract)
Background Synonyms: SEMA3E, SEMAH, coll-5, M-SEMAH, M-SemaK, KIAA0331, M-sema H, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E
Gene ID 9723
NCBI Accession NP_036563
Pathways
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 35 kDa and 20 kDa in Human Prostate lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 89.2 kDa according to NP_036563.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Adrenal Medulla shows granular cytoplasmic staining increasing towards the zona reticularis. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E) (C-Term) antibody (ABIN185359) ABIN185359 (2.5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed anti...
Background publications Gu, Yoshida, Livet et al.: "Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins." in: Science (New York, N.Y.), Vol. 307, Issue 5707, pp. 265-8, 2005 (PubMed).

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